Ruminococcus albus 7: Difference between revisions
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'''''Ruminococcus albus''''' '''7 (DSM 20455 ATCC 27210 7 JCM 14654)''' | '''''Ruminococcus albus''''' '''7 (DSM 20455 ATCC 27210 7 JCM 14654)''' | ||
''Ruminococcus albus'' | ''Ruminococcus albus'' 7 is an obligately anaerobic, Gram-positive bacterium first isolated from the bovine rumen by Robert Edward Hungate in 1951 [1]. This strain primarily utilizes plant-derived carbohydrates, such as cellulose, xylan, and cellobiose, as fermentation substrates, and produces ethanol and CO<sub>2</sub> as it primary fermentation end products, along with relatively law amount of acetate [2]. | ||
This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen. Additioally, the production of H<sub>2</sub> during fermentation by ''R. albus'', providing electrons for CO₂ reduction and methane production by methanogenic archaea [3]. Consequently, understanding the metabolic pathways and symbiotic relationships of ''R. albus'' 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock. | This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen. Additioally, the production of H<sub>2</sub> during fermentation by ''R. albus'', providing electrons for CO₂ reduction and methane production by methanogenic archaea [3]. Consequently, understanding the metabolic pathways and symbiotic relationships of ''R. albus'' 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock. | ||
Revision as of 22:20, 29 October 2026
Introduction
Ruminococcus albus 7 (DSM 20455 ATCC 27210 7 JCM 14654)
Ruminococcus albus 7 is an obligately anaerobic, Gram-positive bacterium first isolated from the bovine rumen by Robert Edward Hungate in 1951 [1]. This strain primarily utilizes plant-derived carbohydrates, such as cellulose, xylan, and cellobiose, as fermentation substrates, and produces ethanol and CO2 as it primary fermentation end products, along with relatively law amount of acetate [2].
This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen. Additioally, the production of H2 during fermentation by R. albus, providing electrons for CO₂ reduction and methane production by methanogenic archaea [3]. Consequently, understanding the metabolic pathways and symbiotic relationships of R. albus 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock.
LPSN: Ruminococcus albus Hungate 1957
NCBI: Ruminococcus albus
GTDB: Ruminococcus albus
Risk group: 1
Global Methane Hub Metadata
| Origin: | United States of America, Macike lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| G(6mA)TC | |
| GCGC(6mA)G | |
| CAG(6mA)KC | |
| CTYR(6mA)G | |
| C(6mA)GNNNNNNNTTYG/
CRA(6mA)NNNNNNNCTG |
|
| G(6mA)STC | |
| GAGG(6mA)G | |
| G(5mC)WGC | |
| GGN(5mC)C | |
| CTC(4mC)TC |
Methylating E. coli
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Culturing Conditions
R. albus 7 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. albus 7 on M10 agar is close to 100%.
Antimicrobial MIC Tests
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
| Antibiotic | Concentration (µg/ml) |
|---|---|
| Ampicilin | 2.92 |
| Chlorhamphenicol | 0.08 |
| Erythromycin | 1.79 |
| Gentamycin | 4.67 |
| Kanamycin | 5.77 |
| Streptomycin | Resistance (>200) |
| Tetracycline | 1.0 |
Protocols
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Transformation Results
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External Links
[1] Hungate RE. 1957. Microorganisms in the rumen of cattle fed a constant ration. Can J Microbiol 3:289–311.
[2] Pavlostathis SG, Miller TL, Wolin MJ. 1988. Fermentation of Insoluble Cellulose by Continuous Cultures of Ruminococcus albus. Appl Environ Microbiol 54:2655–2659.
[3] Kim NK, Koike S, Kelly W, Attwood GT, Cook GM, Cann I, Mackie RI. 2026. Transcriptomic and metabolic analyses of interspecies hydrogen and electron transfer during syntrophic growth of Ruminococcus albus 7 with four different hydrogen-utilizing Methanobrevibacter strains. Anim Microbiome https://doi.org/10.1186/s42523-026-00644-8.
