Ruminococcus productus B43: Difference between revisions
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[[File:B43SEM.jpg|thumb|Scanning electron microscopy image of ''Blautia producta'' B43. Source: Yuqi Li]] | |||
== Introduction == | == Introduction == | ||
Latest revision as of 22:05, 30 October 2026
Introduction
Blautia producta B43 (Ruminococcus productus B43) is a strictly anaerobic, Gram-positive bacterium belonging to the family Lachnospiraceae. The species was previously classified as Peptostreptococcus productus and later as Ruminococcus productus before being transferred to the genus Blautia by Liu et al. in 2008 [1, 2]. Members of the genus Blautia are commonly found in the gastrointestinal tracts of humans and animals and are involved in carbohydrate fermentation and short-chain fatty acid production [3].
B. producta is a metabolically versatile anaerobic bacterium associated with carbohydrate fermentation. Members of this species can produce organic acids during fermentation, contributing to microbial carbon metabolism in anaerobic environments [3]. The genus Blautia has also attracted attention for its potential roles in gut microbial metabolism and host–microbe interactions [3].
LPSN: Blautia producta (Prévot 1941) Liu et al. 2008.
NCBI: Blautia producta (Taxonomy ID: 33035).
GTDB: Taxonomic assignment of B43 requires genome-based confirmation.
Type strain: JCM 1471T (= DSM 2950 = ATCC 27340).
Risk group: 2
Global Methane Hub Metadata
| Origin: | Illinois, Mackie Lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| MIC and Culturing Conditions by: | Yuqi Li |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| (6mA)YCNNNNNNGRT | 42.5 |
| GAT(5mC) | 93.5 |
| G(5mC)CGGC | 98.83 |
Methylating E. coli Strains
Culturing Conditions
Blautia producta B43 grows in M2GSC medium at 37 °C under strictly anaerobic conditions. Cultures are maintained in an anaerobic chamber containing 95% CO2 and 5% H2.
Antibiotic MIC Tests
| Antibiotic | Concentration (μg/mL) |
|---|---|
| Ampicillin | Resistant (>200 μg/mL) |
| Kanamycin | Resistant (>200 μg/mL) |
| Chloramphenicol | 0.69 |
| Tetracycline | 0.10 |
| Gentamicin | 6.02 |
| Streptomycin | 2.9 |
| Erythromycin | 1.09 |
Protocols
Transformation Results
External Links
[1] Liu C, Finegold SM, Song Y, Lawson PA. 2008. Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 58: 1896–1902. doi: 10.1099/ijs.0.65208-0.
[2] LPSN – List of Prokaryotic names with Standing in Nomenclature. Blautia producta (Prévot 1941) Liu et al. 2008. https://lpsn.dsmz.de/species/blautia-producta?utm_source
[3] Liu X, Mao B, Gu J, et al. 2021. Blautia—a new functional genus with potential probiotic properties? Gut Microbes 13: 1–21. doi: 10.1080/19490976.2021.1875796.
