Acetitomaculum ruminis 139BT: Difference between revisions
No edit summary |
|||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
[[File:139BSEM.jpg|thumb|Scanning electron microscopy (SEM) image of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]] | |||
== Introduction == | == Introduction == | ||
'''''Acetitomaculum ruminis'' 139B<sup>T</sup>''' (DSM 5522, ATCC 43876) is a strictly anaerobic, Gram-positive, rod-shaped bacterium belonging to the family Lachnospiraceae. It was originally isolated from the rumen of cattle and first described by Greening and Leedle in 1989 [1]. The strain 139B<sup>T</sup> is designated as the type strain of ''A. ruminis'' [1, 2]. | |||
''A. ruminis'' is an acetogenic bacterium capable of utilizing hydrogen (H<sub>2</sub>) and carbon dioxide (CO<sub>2</sub>) to produce acetate through the Wood–Ljungdahl pathway [1, 3]. It can also utilize organic substrates for growth. As a hydrogen-consuming acetogen, ''A. ruminis'' may compete with methanogenic archaea for hydrogen in the rumen. However, methanogenesis is generally considered the dominant hydrogen disposal pathway under typical rumen conditions [3, 4]. | |||
The genome sequence of ''A. ruminis'' 139B<sup>T</sup> is available through the Hungate1000 project, which provides genomic resources for understanding the metabolic capabilities and ecological roles of rumen microorganisms [5]. Its acetogenic metabolism makes this bacterium particularly interesting for studying rumen hydrogen utilization and potential alternative pathways to methane formation. | |||
LPSN: ''Acetitomaculum ruminis'' (Greening and Leedle, 1989). | |||
NCBI: ''Acetitomaculum ruminis''. | |||
GTDB: ''Acetitomaculum ruminis''. | |||
Type strain: 139B<sup>T</sup> (= DSM 5522 = ATCC 43876). | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
| Line 59: | Line 74: | ||
|} | |} | ||
== Methylating E. coli Strains == | == Methylating ''E. coli'' Strains == | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''Acetitomaculum ruminis'' 139B<sup>T</sup> can be cultivated in [[M2GSC Medium|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>. | |||
== Antibiotic MIC Tests == | == Antibiotic MIC Tests == | ||
[[File:139BMIC.png|thumb|MIC tests of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li|left]] | |||
{| class="wikitable" | |||
!Antibiotic | |||
!Concentration ('''μ'''g/mL) | |||
|- | |||
|Ampicillin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Kanamycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Chloramphenicol | |||
|1.03 | |||
|- | |||
|Tetracycline | |||
|0.19 | |||
|- | |||
|Gentamicin | |||
|4.75 | |||
|- | |||
|Streptomycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Erythromycin | |||
|2.67 | |||
|} | |||
== Protocols == | == Protocols == | ||
| Line 70: | Line 112: | ||
== External Links == | == External Links == | ||
[1] Greening RC, Leedle JAZ. 1989. Enrichment and isolation of ''Acetitomaculum ruminis'', gen. nov., sp. nov.: acetogenic bacteria from the bovine rumen. Archives of Microbiology 151: 399–406. doi: 10.1007/BF00416597. | |||
[2] DSMZ – German Collection of Microorganisms and Cell Cultures. ''Acetitomaculum ruminis'' DSM 5522. <nowiki>https://www.dsmz.de/collection/catalogue/details/culture/DSM-5522</nowiki>. | |||
[3] Ragsdale SW, Pierce E. 2008. Acetogenesis and the Wood–Ljungdahl pathway of CO<sub>2</sub> fixation. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 1784: 1873–1898. doi: 10.1016/j.bbapap.2008.08.012. | |||
[4] Ungerfeld EM. 2020. Metabolic hydrogen flows in rumen fermentation: principles and possibilities of interventions. Frontiers in Microbiology 11: 589. doi: 10.3389/fmicb.2020.00589. | |||
[5] Seshadri R, Leahy SC, Attwood GT, et al. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnology 36: 359–367. doi: 10.1038/nbt.4110. | |||
Latest revision as of 18:26, 30 October 2026
Introduction
Acetitomaculum ruminis 139BT (DSM 5522, ATCC 43876) is a strictly anaerobic, Gram-positive, rod-shaped bacterium belonging to the family Lachnospiraceae. It was originally isolated from the rumen of cattle and first described by Greening and Leedle in 1989 [1]. The strain 139BT is designated as the type strain of A. ruminis [1, 2].
A. ruminis is an acetogenic bacterium capable of utilizing hydrogen (H2) and carbon dioxide (CO2) to produce acetate through the Wood–Ljungdahl pathway [1, 3]. It can also utilize organic substrates for growth. As a hydrogen-consuming acetogen, A. ruminis may compete with methanogenic archaea for hydrogen in the rumen. However, methanogenesis is generally considered the dominant hydrogen disposal pathway under typical rumen conditions [3, 4].
The genome sequence of A. ruminis 139BT is available through the Hungate1000 project, which provides genomic resources for understanding the metabolic capabilities and ecological roles of rumen microorganisms [5]. Its acetogenic metabolism makes this bacterium particularly interesting for studying rumen hydrogen utilization and potential alternative pathways to methane formation.
LPSN: Acetitomaculum ruminis (Greening and Leedle, 1989).
NCBI: Acetitomaculum ruminis.
GTDB: Acetitomaculum ruminis.
Type strain: 139BT (= DSM 5522 = ATCC 43876).
Global Methane Hub Metadata
| Origin: | Illinois, Mackie Lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| MIC and Culturing Conditions by: | Yuqi Li |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| CTGC(6mA)G | 95.68% |
| TA(6mA)GNNNNNNTCC | 95.61% |
| GG(6mA)NNNNNNCTTA | 96.1% |
| G(6mA)TGNNNNNNTCC | 84.95% |
| GG(6mA)NNNNNNCATT | 92.38% |
| G(6mA)TCG | 89.82% |
| A(6mA)TGNNNNNNTCC | 96.19% |
| GG(6mA)NNNNNNCATT | 90.36% |
| GAT(5mC) | 32.66% |
Methylating E. coli Strains
Culturing Conditions
Acetitomaculum ruminis 139BT can be cultivated in M2GSC medium at 37 °C under strictly anaerobic conditions. Cultures are maintained in an anaerobic chamber containing 95% CO2 and 5% H2.
Antibiotic MIC Tests
| Antibiotic | Concentration (μg/mL) |
|---|---|
| Ampicillin | Resistant (>200 μg/mL) |
| Kanamycin | Resistant (>200 μg/mL) |
| Chloramphenicol | 1.03 |
| Tetracycline | 0.19 |
| Gentamicin | 4.75 |
| Streptomycin | Resistant (>200 μg/mL) |
| Erythromycin | 2.67 |
Protocols
Transformation Results
External Links
[1] Greening RC, Leedle JAZ. 1989. Enrichment and isolation of Acetitomaculum ruminis, gen. nov., sp. nov.: acetogenic bacteria from the bovine rumen. Archives of Microbiology 151: 399–406. doi: 10.1007/BF00416597.
[2] DSMZ – German Collection of Microorganisms and Cell Cultures. Acetitomaculum ruminis DSM 5522. https://www.dsmz.de/collection/catalogue/details/culture/DSM-5522.
[3] Ragsdale SW, Pierce E. 2008. Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 1784: 1873–1898. doi: 10.1016/j.bbapap.2008.08.012.
[4] Ungerfeld EM. 2020. Metabolic hydrogen flows in rumen fermentation: principles and possibilities of interventions. Frontiers in Microbiology 11: 589. doi: 10.3389/fmicb.2020.00589.
[5] Seshadri R, Leahy SC, Attwood GT, et al. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnology 36: 359–367. doi: 10.1038/nbt.4110.
