Ruminococcus albus 8: Difference between revisions
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[[File:Image2.png|thumb|350x350px|Scanning electron microscopic image of ''Ruminococcus albus'' 8. | |||
<small>This photo belongs to the GMH Project and was taken by Kingsley Boateng.</small>]] | |||
== Introduction == | == Introduction == | ||
'''''Ruminococcus albus''''' '''8''' | |||
''Ruminococcus albus'' 8 is an obligately anaerobic, fibrolytic bacterium originally isolated from the rumen of an alfalfa-fed cow by Marvin P. Bryant. | |||
While ''R. albus'' 8 shares core phenotypic characteristics with other strains, genomic and transcriptomic analyses indicate that it exhibits a specialized ecological preference for hemicellulose and xylan degradation over highly crystalline cellulose, distinguishing it from the highly cellulolytic ''R. albus'' 7 [1]. The strain secretes multiple endoxylanases and utilizes a unique phosphoketolase pathway for the rapid catabolism of pentose sugars, producing acetate, ethanol, formate, and hydrogen [2]. In the rumen, ''R. albus'' 8 contributes to the energy acquisition of the host animal through the production of volatile fatty acids, while acting in syntrophy with methanogenic archaea by providing the hydrogen necessary for enteric methane production. | |||
LPSN: ''Ruminococcus albus'' Hungate 1957 | |||
NCBI: ''Ruminococcus albus'' 8 | |||
GTDB: ''Ruminococcus albus'' | |||
Risk group: 1 | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
| Line 7: | Line 21: | ||
|+ | |+ | ||
|Origin: | |Origin: | ||
|United States of America, | |United States of America, Mackie lab | ||
|- | |- | ||
|Sequenced by: | |Sequenced by: | ||
| Line 19: | Line 33: | ||
|- | |- | ||
|Methyltransferases cloned by: | |Methyltransferases cloned by: | ||
| | |Roy Rodriguez Carrero | ||
|- | |- | ||
|MIC and Culturing Conditions by: | |MIC and Culturing Conditions by: | ||
| | |Roy Rodriguez Carrero | ||
|- | |- | ||
|Transformation by: | |Transformation by: | ||
| Line 29: | Line 43: | ||
== Genome == | == Genome == | ||
[[File:2N52CS 1 R8 1 circos.png|center|thumb|300x300px|Circos plot of ''R. albus'' 8. Source: Makoto Adachi]] | |||
== DefenseFinder Results == | == DefenseFinder Results == | ||
== Methylome == | == Methylome == | ||
{| class="wikitable" | |||
!Motif | |||
!Percent methylated | |||
|- | |||
|G(6mA)TCC | |||
|99.53 | |||
|- | |||
|GG(6mA)TC | |||
|99.93 | |||
|- | |||
|GG(6mA)GG | |||
|98.88 | |||
|- | |||
|GNC(6mA)YNNNNNNCCT | |||
|97.47 | |||
|- | |||
|(6mA)GGNNNNNNRTGNC | |||
|99.28 | |||
|- | |||
|G(6mA)TNNNNCTCA | |||
|98.36 | |||
|- | |||
|TG(6mA)GNNNNATC | |||
|97.64 | |||
|- | |||
|A(5mC)GGG | |||
|97.16 | |||
|- | |||
|C(5mC)CGT | |||
|87.25 | |||
|- | |||
|G(5mC)CTCC | |||
|85.17 | |||
|} | |||
== Methylating ''E. coli'' == | == Methylating ''E. coli'' == | ||
| Line 41: | Line 87: | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''R. albus'' 8 grown well in [[M2GSC Medium|M2GSC]] and [[M10 Medium|M10]] medium at 37°C. Plating efficiency of ''R. albus'' 8 on M10 agar is close to 89%. | |||
== Antimicrobial MIC Tests == | == Antimicrobial MIC Tests == | ||
Note: Antimicrobial MIC was assessed using [[M2GSC Medium|M2GSC]] liquid medium. | |||
{| class="wikitable" | |||
|+ | |||
!'''Antibiotic''' | |||
!'''Concentration (µg/ml)''' | |||
|- | |||
|Ampicilin | |||
|0.1 | |||
|- | |||
|Chlorhamphenicol | |||
|Resistance (>200) | |||
|- | |||
|Erythromycin | |||
|0.1 | |||
|- | |||
|Gentamycin | |||
|0.1 | |||
|- | |||
|Kanamycin | |||
|0.6 | |||
|- | |||
|Streptomycin | |||
|Resistance (>200) | |||
|- | |||
|Tetracycline | |||
|1 | |||
|} | |||
== Protocols == | == Protocols == | ||
| Line 53: | Line 125: | ||
== External Links == | == External Links == | ||
[1] Kwon I. 2016. Comparative whole genome transcriptional responses of ''Ruminococcus albus'' strain 7 and 8: a case for specialization and niche differentiation. Ph.D. dissertation. University of Illinois at Urbana-Champaign, Urbana, IL. | |||
[2] Moon YH, Iakiviak M, Bauer S, Mackie RI, Cann IKO. 2011. Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes. Appl Environ Microbiol 77:5157–5169. | |||
Latest revision as of 18:17, 30 October 2026
Introduction
Ruminococcus albus 8
Ruminococcus albus 8 is an obligately anaerobic, fibrolytic bacterium originally isolated from the rumen of an alfalfa-fed cow by Marvin P. Bryant.
While R. albus 8 shares core phenotypic characteristics with other strains, genomic and transcriptomic analyses indicate that it exhibits a specialized ecological preference for hemicellulose and xylan degradation over highly crystalline cellulose, distinguishing it from the highly cellulolytic R. albus 7 [1]. The strain secretes multiple endoxylanases and utilizes a unique phosphoketolase pathway for the rapid catabolism of pentose sugars, producing acetate, ethanol, formate, and hydrogen [2]. In the rumen, R. albus 8 contributes to the energy acquisition of the host animal through the production of volatile fatty acids, while acting in syntrophy with methanogenic archaea by providing the hydrogen necessary for enteric methane production.
LPSN: Ruminococcus albus Hungate 1957
NCBI: Ruminococcus albus 8
GTDB: Ruminococcus albus
Risk group: 1
Global Methane Hub Metadata
| Origin: | United States of America, Mackie lab |
| Sequenced by: | Makoto Adachi |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| G(6mA)TCC | 99.53 |
| GG(6mA)TC | 99.93 |
| GG(6mA)GG | 98.88 |
| GNC(6mA)YNNNNNNCCT | 97.47 |
| (6mA)GGNNNNNNRTGNC | 99.28 |
| G(6mA)TNNNNCTCA | 98.36 |
| TG(6mA)GNNNNATC | 97.64 |
| A(5mC)GGG | 97.16 |
| C(5mC)CGT | 87.25 |
| G(5mC)CTCC | 85.17 |
Methylating E. coli
add here
Culturing Conditions
R. albus 8 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. albus 8 on M10 agar is close to 89%.
Antimicrobial MIC Tests
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
| Antibiotic | Concentration (µg/ml) |
|---|---|
| Ampicilin | 0.1 |
| Chlorhamphenicol | Resistance (>200) |
| Erythromycin | 0.1 |
| Gentamycin | 0.1 |
| Kanamycin | 0.6 |
| Streptomycin | Resistance (>200) |
| Tetracycline | 1 |
Protocols
add here
Transformation Results
add here
External Links
[1] Kwon I. 2016. Comparative whole genome transcriptional responses of Ruminococcus albus strain 7 and 8: a case for specialization and niche differentiation. Ph.D. dissertation. University of Illinois at Urbana-Champaign, Urbana, IL.
[2] Moon YH, Iakiviak M, Bauer S, Mackie RI, Cann IKO. 2011. Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes. Appl Environ Microbiol 77:5157–5169.
