Prevotella bryantii B14: Difference between revisions
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[[File:B14SEM.jpg|thumb|Scanning electron microscopy image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology. Source: Yuqi Li]] | |||
== Introduction == | == Introduction == | ||
'''''Segatella bryantii'' B<sub>1</sub>4''' | '''''Segatella bryantii'' B<sub>1</sub>4''' | ||
''Segatella bryantii'' B<sub>1</sub>4 (DSM 11371), formerly ''Prevotella bryantii'', was isolated from rumen contents of a Holstein cow fed alfalfa hay in the USA [1]. It was originally named as ''Bacteroides ruminicola'' subsp. ''brevis'' and subsequently changed to ''Prevotella bryantii'' [2]. The strain was originally designated B<sub>1</sub>4 but | ''Segatella bryantii'' B<sub>1</sub>4 (DSM 11371), formerly ''Prevotella bryantii'', was isolated from rumen contents of a Holstein cow fed alfalfa hay in the USA [1]. It was originally named as ''Bacteroides ruminicola'' subsp. ''brevis'' and subsequently changed to ''Prevotella bryantii'' [2]. The strain was originally designated B<sub>1</sub>4 but B<sub>1</sub>4 is commonly used. B<sub>1</sub>4 has been the focus of many studies and is able to ferment a wide range of carbohydrates [3, 4]. The genes involved in xylan degradation have been characterized [5]. | ||
Taxonomic analysis of the genus ''Prevotella'' lists B14 as the ''Segatella bryantii'' type strain [6]. However, NCBI now records B14 and C21a as belonging to the species ''Segatella baroniae'', although the reason for this is unclear. | Taxonomic analysis of the genus ''Prevotella'' lists B14 as the ''Segatella bryantii'' type strain [6]. However, NCBI now records B14 and C21a as belonging to the species ''Segatella baroniae'', although the reason for this is unclear. | ||
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== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
{| class="wikitable" | |||
|Origin: | |||
|Illinois, Mackie Lab | |||
|- | |||
|Sequenced by: | |||
|Yuqi Li | |||
|- | |||
|Assembled by: | |||
|William G. Alexander | |||
|- | |||
|Methylome called by: | |||
|William G. Alexander | |||
|- | |||
|MIC and Culturing Conditions by: | |||
|Yuqi Li | |||
|} | |||
== Genome == | == Genome == | ||
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== Culturing Conditions == | == Culturing Conditions == | ||
''Prevotella bryantii'' B<sub>1</sub>4 grows well in [[M2GSC Medium|M2GSC]] or M2G medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in either medium. B<sub>1</sub>4 can also be grown on M10G agar plates, with colonies typically appearing after 36–72 h of incubation. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>. | |||
== Antimicrobial MIC Tests == | == Antimicrobial MIC Tests == | ||
[[File:Data 2.jpg|thumb|MIC tests of ''P. bryantii'' B<sub>1</sub>4. Source: Yuqi Li|left]] | |||
{| class="wikitable" | |||
!Antibiotic | |||
!Concentration ('''μ'''g/mL) | |||
|- | |||
|Ampicillin | |||
|0.52 | |||
|- | |||
|Kanamycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Chloramphenicol | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Tetracycline | |||
|0.20 | |||
|- | |||
|Gentamicin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Streptomycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Erythromycin | |||
|4.3 | |||
|} | |||
== Protocols == | == Protocols == | ||
Latest revision as of 17:18, 30 October 2026
Introduction
Segatella bryantii B14
Segatella bryantii B14 (DSM 11371), formerly Prevotella bryantii, was isolated from rumen contents of a Holstein cow fed alfalfa hay in the USA [1]. It was originally named as Bacteroides ruminicola subsp. brevis and subsequently changed to Prevotella bryantii [2]. The strain was originally designated B14 but B14 is commonly used. B14 has been the focus of many studies and is able to ferment a wide range of carbohydrates [3, 4]. The genes involved in xylan degradation have been characterized [5].
Taxonomic analysis of the genus Prevotella lists B14 as the Segatella bryantii type strain [6]. However, NCBI now records B14 and C21a as belonging to the species Segatella baroniae, although the reason for this is unclear.
Draft genome sequences are available for B14 (3.6 Mb) [7]. Resequencing indicates that Segatella bryantii strains (B14 and C21a) each have two replicons of ~2.5 and ~1.0 Mb. Two other sequenced P. bryantii cultures (TC1-1, TS1-5) have a similar genomic architecture suggesting this is a feature of this species.
The B14 genome encodes a large conjugative transposon [8] although this has not been characterized in detail or shown to be transferable. Based on the presence of a the traG gene (TIGR03783) large conjugative transposons may be encoded by other rumen Prevotella strains.
LPSN: Segatella bryantii (Avguštin et al. 1997) Hitch et al. 2023.
NCBI: Segatella baroniae.
GTBD: Prevotella bryantii.
Risk group: 2.
Global Methane Hub Metadata
| Origin: | Illinois, Mackie Lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| MIC and Culturing Conditions by: | Yuqi Li |
Genome
DefenseFinder Results
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Methylome
| Motif | Percent methylated |
|---|---|
| GTC(6mA)NNNNNRTC/G(6mA)YNNNNNTGAC | 75.00%/80.51% |
| GTHN(6mA)NCC | 76.99% |
| GTC(6mA)G | 71.12% |
| A(6mA)CNNNNNGGG/C(4mC)CNNNNNGTT | 82.78%/ |
| GG(5mC)GT | 82.25% |
| A(5mC)GCC | 81.20% |
Methylating E. coli
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Culturing Conditions
Prevotella bryantii B14 grows well in M2GSC or M2G medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in either medium. B14 can also be grown on M10G agar plates, with colonies typically appearing after 36–72 h of incubation. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO2 and 5% H2.
Antimicrobial MIC Tests
| Antibiotic | Concentration (μg/mL) |
|---|---|
| Ampicillin | 0.52 |
| Kanamycin | Resistant (>200 μg/mL) |
| Chloramphenicol | Resistant (>200 μg/mL) |
| Tetracycline | 0.20 |
| Gentamicin | Resistant (>200 μg/mL) |
| Streptomycin | Resistant (>200 μg/mL) |
| Erythromycin | 4.3 |
Protocols
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Transformation Results
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External Links
[1] Bryant MP, Small N, Bouma C, Chu H. 1958. Bacteroides ruminicola n. sp. and Succinimonas amylolytica; the new genus and species. Species of succinic acid-producing anaerobic bacteria of the bovine rumen. J Bacteriol 76: 15-23. doi: 10.1128/jb.76.1.15-23.1958.
[2] Avgustin G, Wallace RJ, Flint HJ. 1997. Phenotypic diversity among ruminal isolates of Prevotella ruminicola: proposal of Prevotella brevis sp. nov., Prevotella bryantii sp. nov., and Prevotella albensis sp. nov. and redefinition of Prevotella ruminicola. Int J Syst Bacteriol 47: 284-8. doi: 10.1099/00207713-47-2-284.
[3] Accetto T, Avguštin G. 2019. The diverse and extensive plant polysaccharide degradative apparatuses of the rumen and hindgut Prevotella species: A factor in their ubiquity? Syst Appl Microbiol 42: 107-116. doi: 10.1016/j.syapm.2018.10.001.
[4] https://bacdive.dsmz.de/strain/12536.
[5] Dodd D, Mackie RI, Cann IK. 2011. Xylan degradation, a metabolic property shared by rumen and human colonic Bacteroidetes. Mol Microbiol 79: 292-304. doi: 10.1111/j.1365-2958.2010.07473.x.
[6] Hitch TCA, Bisdorf K, Afrizal A, Riedel T, Overmann J, Strowig T, Clavel T. 2022. A taxonomic note on the genus Prevotella: Description of four novel genera and emended description of the genera Hallella and Xylanibacter. Syst Appl Microbiol 45: 126354. doi: 10.1016/j.syapm.2022.126354.
[7] Purushe J, Fouts DE, Morrison M, White BA, Mackie RI; North American Consortium for Rumen Bacteria; Coutinho PM, Henrissat B, Nelson KE. 2010. Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche. Microb Ecol 60: 721-9. doi: 10.1007/s00248-010-9692-8.
[8] Gorenc K, Accetto T, Avguštin G. 2012. Bioinformatic evidence and characterization of novel putative large conjugative transposons residing in genomes of genera Bacteroides and Prevotella. Folia Microbiol (Praha) 57: 285-90. doi: 10.1007/s12223-012-0126-5.
