Butyrivibrio hungatei MB2003: Difference between revisions
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[[File:MB2003SEM.jpg|thumb|Scanning electron microscopy (SEM) image of ''Butyrivibrio hungatei'' MB2003. Source: Yuqi Li]] | |||
== Introduction == | == Introduction == | ||
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== Methylating ''E. coli'' == | |||
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== Culturing Conditions == | |||
''Butyrivibrio hungatei'' MB2003 grows well in [[M2GSC Medium|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in [[M2GSC Medium|M2GSC]] medium. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>. | |||
== Antibiotic MIC Tests == | |||
[[File:MICMB2003.jpg|left|thumb|MIC tests of ''Butyrivibrio hungatei'' MB2003. Source: Yuqi Li]] | |||
{| class="wikitable" | |||
!Antibiotic | |||
!Concentration ('''μ'''g/mL) | |||
|- | |||
|Ampicillin | |||
|0.91 | |||
|- | |||
|Kanamycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Chloramphenicol | |||
|0.59 | |||
|- | |||
|Tetracycline | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Gentamicin | |||
|2.08 | |||
|- | |||
|Streptomycin | |||
|Resistant (>200 μg/mL) | |||
|- | |||
|Erythromycin | |||
|4.18 | |||
|} | |||
== External Links == | == External Links == | ||
Latest revision as of 17:13, 30 October 2026
Introduction
Butyrivibrio hungatei MB2003
Butyrivibrio hungatei MB2003 (DSM 120820) was isolated from the plant-adherent fraction of bovine rumen contents in NZ [1, cow B, 2]. It ferments a range of carbohydrates but has a smaller genome and a more limited repertoire of carbohydrate active enzymes than other Butyrivibrio species (81 glycosyl hydrolases, 86 glycosyl transferases, 3 polysaccharide lyases, 8 carbohydrate esterases) [3, 4].
The MB2003 genome (3,386,314 bp) consists of a chromosome (3,143,784 bp) and three plasmids (144,470 bp, 91,776 bp and 6,284 bp) [5].
LPSN: Butyrivibrio hungatei Kopecný et al. 2003
NCBI: Butyrivibrio hungatei.
GTDB: Butyrivibrio hungatei. Groups with the type strain of B. hungatei (JK615T, DSM 14810) [6]
Risk group: 1
Global Methane Hub Metadata
| Origin: | New Zealand, Kelly Lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Yuqi Li |
Genome
Methylome
| Motif | Percent methylated |
|---|---|
| C(6mA)CNNNNNNTGTT/
AAC(6mA)NNNNNNGTG |
94.54%/94.83% |
| ATTA(6mA)T | 97.76% |
Methylating E. coli
add here
Culturing Conditions
Butyrivibrio hungatei MB2003 grows well in M2GSC medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in M2GSC medium. Cultures are maintained in an anaerobic chamber containing 95% CO2 and 5% H2.
Antibiotic MIC Tests
| Antibiotic | Concentration (μg/mL) |
|---|---|
| Ampicillin | 0.91 |
| Kanamycin | Resistant (>200 μg/mL) |
| Chloramphenicol | 0.59 |
| Tetracycline | Resistant (>200 μg/mL) |
| Gentamicin | 2.08 |
| Streptomycin | Resistant (>200 μg/mL) |
| Erythromycin | 4.18 |
External Links
[1] Noel S. 2013. Cultivation and community composition analysis of plant-adherent rumen bacteria. PhD dissertation. Massey University, Palmerston North, New Zealand. https://mro.massey.ac.nz/server/api/core/bitstreams/bcfbe4cb-19a9-40c0-b5e8-61f9ddb90ce0/content.
[2] https://bacdive.dsmz.de/strain/176811
[3] Palevich N, Kelly WJ, Ganesh S, Rakonjac J, Attwood GT. 2019. Butyrivibrio hungatei MB2003 competes effectively for soluble sugars released by Butyrivibrio proteoclasticus B316T during growth on xylan or pectin. Appl Environ Microbiol 85: e02056-18. doi: 10.1128/AEM.02056-18.
[4] https://www.cazy.org/b7466.html
[5] Palevich N, Kelly WJ, Leahy SC, Altermann E, Rakonjac J, Attwood GT. 2017. The complete genome sequence of the rumen bacterium Butyrivibrio hungatei MB2003. Stand Genomic Sci 12: 72. doi: 10.1186/s40793-017-0285-8.
[6] Kopecny J, Zorec M, Mrazek J, Kobayashi Y, Marinsek-Logar R. 2003. Butyrivibrio hungatei sp. nov. and Pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen. Int J Syst Evol Microbiol 53: 201–9. doi: 10.1099/ijs.0.02345-0.
