Streptococcus bovis JB1: Difference between revisions
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[[File:S. bovis SEM.jpg|thumb|350x350px|Scanning electron micrograph of ''Streptococcus bovis'' JB1 by Ahmed Hetta]] | |||
== Introduction == | == Introduction == | ||
''Streptococcus bovis'' JB1 was isolated from a cow fed alfalfa hay in Davis, CA, USA[1]. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants. | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
{| class="wikitable" | |||
|+ | |||
|Origin: | |||
|Illinois, Mackie Lab | |||
|- | |||
|Sequenced by: | |||
|Ahmed Hetta and Yuqi Li | |||
|- | |||
|Assembled by: | |||
|William G. Alexander | |||
|- | |||
|Methylome called by: | |||
|William G. Alexander | |||
|- | |||
|Methyltransferases cloned by: | |||
|No methylation motifs have been detected | |||
|- | |||
|MIC and Culturing Conditions by: | |||
|Ahmed Hetta and Yuqi Li | |||
|- | |||
|Transformation by: | |||
|Ahmed Hetta | |||
|} | |||
== Genome == | == Genome == | ||
[[File:Streptococcus_bovis_JB1_1_1_circos.png|center|thumb|Circos plot of ''Streptococcus bovis'' JB1|400x400px]] | |||
== DefenseFinder Results == | == DefenseFinder Results == | ||
== Methylome == | == Methylome == | ||
No methylation motifs were detected in ''Streptococcus bovis'' JB1. | |||
== Methylating ''E. coli'' | == Methylating E. coli Strains == | ||
No ''E. coli'' methylation strain has been constructed for ''S. bovis'' JB1 strain | |||
== Culturing Conditions == | == Culturing Conditions == | ||
''Streptococcus bovis'' JB1 grows on both M2GSC and [[SDM culture medium|SDM]] (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>. Similarly, ''Streptococcus bovis'' JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>. | |||
== | == Antibiotic MIC Tests == | ||
[[File:Streptococcus_bovis_JB1_MIC.png|left|thumb|MIC tests of ''Streptococcus bovis'' JB1|375x375px]] | |||
{| class="wikitable" | |||
!antibiotic | |||
!concentration (ug/mL) | |||
|- | |||
|Ampicillin | |||
|0.25 | |||
|- | |||
|Kanamycin | |||
|Resistant (>200 ug/mL) | |||
|- | |||
|Chloramphenicol | |||
|0.50 | |||
|- | |||
|Tetracycline | |||
|0.23 | |||
|- | |||
|Gentamicin | |||
|1.0 | |||
|- | |||
|Streptomycin | |||
|Resistant (>200 ug/mL) | |||
|- | |||
|Erythromycin | |||
|1 | |||
|} | |||
== Protocols == | == Protocols == | ||
* [[SDM culture medium|Preparation of SDM culture medium]] | |||
* [[M2GSC Medium|Preparation of M2GSC culture medium]] | |||
* [[Streptococcus transformation protocol|Streptococcus transformation '''protocol''']] | |||
== Transformation Results == | == Transformation Results == | ||
== External Links == | == External Links == | ||
# arvis GN, Kurtovic A, Hay AG, Russell JB. The physiological and genetic diversity of bovine Streptococcus bovis strains(1). FEMS Microbiol Ecol. 2001 Mar;35(1):49-56. doi: 10.1016/s0168-6496(00)00110-0. PMID: 11248389. | |||
Latest revision as of 17:09, 30 October 2026
Introduction
Streptococcus bovis JB1 was isolated from a cow fed alfalfa hay in Davis, CA, USA[1]. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.
Global Methane Hub Metadata
| Origin: | Illinois, Mackie Lab |
| Sequenced by: | Ahmed Hetta and Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | No methylation motifs have been detected |
| MIC and Culturing Conditions by: | Ahmed Hetta and Yuqi Li |
| Transformation by: | Ahmed Hetta |
Genome
DefenseFinder Results
Methylome
No methylation motifs were detected in Streptococcus bovis JB1.
Methylating E. coli Strains
No E. coli methylation strain has been constructed for S. bovis JB1 strain
Culturing Conditions
Streptococcus bovis JB1 grows on both M2GSC and SDM (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO2 and 5% H2. Similarly, Streptococcus bovis JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO2 and 5% H2.
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Ampicillin | 0.25 |
| Kanamycin | Resistant (>200 ug/mL) |
| Chloramphenicol | 0.50 |
| Tetracycline | 0.23 |
| Gentamicin | 1.0 |
| Streptomycin | Resistant (>200 ug/mL) |
| Erythromycin | 1 |
Protocols
Transformation Results
External Links
- arvis GN, Kurtovic A, Hay AG, Russell JB. The physiological and genetic diversity of bovine Streptococcus bovis strains(1). FEMS Microbiol Ecol. 2001 Mar;35(1):49-56. doi: 10.1016/s0168-6496(00)00110-0. PMID: 11248389.
