Methanobrevibacter boviskoreani JH1T: Difference between revisions
From Global Methane Hub Wikipedia
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== Genome == | == Genome == | ||
Single contig of 2,084,047 bp. | |||
== DefenseFinder Results == | == DefenseFinder Results == | ||
== Methylome == | == Methylome == | ||
{| class="wikitable" | |||
| valign="top" |'''#6mA modified motifs''' | |||
| valign="top" | | |||
| valign="top" | | |||
| valign="top" | | |||
|- | |||
| valign="top" |'''#motif''' | |||
| valign="top" |methylCounts | |||
| valign="top" |totalCounts | |||
| valign="top" |%motifsMethyl | |||
|- | |||
| valign="top" |'''C(6mA)TCC''' | |||
| valign="top" |2452 | |||
| valign="top" |2922 | |||
| valign="top" |83.92 | |||
|- | |||
| valign="top" |'''GATG(6mA)T''' | |||
| valign="top" |2170 | |||
| valign="top" |2556 | |||
| valign="top" |84.90 | |||
|- | |||
| valign="top" |'''GG(6mA)TG''' | |||
| valign="top" |2471 | |||
| valign="top" |2922 | |||
| valign="top" |84.57 | |||
|- | |||
| valign="top" |'''(6mA)GGNNNNNNRTC''' | |||
| valign="top" |1037 | |||
| valign="top" |1342 | |||
| valign="top" |77.27 | |||
|- | |||
| valign="top" |'''G(6mA)YNNNNNNCCT''' | |||
| valign="top" |984 | |||
| valign="top" |1342 | |||
| valign="top" |73.32 | |||
|} | |||
== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''Methanobrevibacter boviskoreani'' JH1T is a strict anaerobe and can be grown in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. boviskoreani JH1T'' requires rumen fluid but does not need volatile fatty acids (VFAs). Upon plating, the strain forms colonies in 7-10 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S . | |||
== Antibiotic MIC Tests == | == Antibiotic MIC Tests == | ||
| Line 44: | Line 80: | ||
!concentration (ug/mL) | !concentration (ug/mL) | ||
|- | |- | ||
| | |Puromycin | ||
| | | | ||
|- | |- | ||
| | |Nourseothricin | ||
| | | | ||
|- | |- | ||
| | |Neomycin | ||
| | | | ||
|- | |- | ||
| | |8-ADP | ||
| | | | ||
|- | |- | ||
| | |5-Fluorouracil | ||
| | | | ||
|- | |- | ||
| | |2-BES | ||
| | | | ||
|- | |- | ||
| | |5-FOA | ||
| | | | ||
|} | |} | ||
Latest revision as of 18:46, 28 October 2026
Introduction
Global Methane Hub Metadata
| Origin: | AgResearch, New Zealand |
| Sequenced by: | Sharik Khan, University of Illinois at Urbana-Champaign |
| Assembled by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Methylome called by: | William G. Alexande, Oak Ridge National Laboratory |
| Methyltransferases cloned by: | Adam Guss, Oak Ridge National Laboratory |
| MIC and Culturing Conditions by: | Sharik Khan, University of Illinois at Urbana-Champaign |
| Transformation by: |
Genome
Single contig of 2,084,047 bp.
DefenseFinder Results
Methylome
| #6mA modified motifs | |||
| #motif | methylCounts | totalCounts | %motifsMethyl |
| C(6mA)TCC | 2452 | 2922 | 83.92 |
| GATG(6mA)T | 2170 | 2556 | 84.90 |
| GG(6mA)TG | 2471 | 2922 | 84.57 |
| (6mA)GGNNNNNNRTC | 1037 | 1342 | 77.27 |
| G(6mA)YNNNNNNCCT | 984 | 1342 | 73.32 |
Methylating E. coli Strains
Culturing Conditions
Methanobrevibacter boviskoreani JH1T is a strict anaerobe and can be grown in both RMO2-medium and MSM (Methanobrevibacter smithii medium) topped with H2/CO2 (80:20, v/v). Growth of M. boviskoreani JH1T requires rumen fluid but does not need volatile fatty acids (VFAs). Upon plating, the strain forms colonies in 7-10 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Puromycin | |
| Nourseothricin | |
| Neomycin | |
| 8-ADP | |
| 5-Fluorouracil | |
| 2-BES | |
| 5-FOA |
