Methanobrevibacter boviskoreani JH1T
From Global Methane Hub Wikipedia
Introduction
Global Methane Hub Metadata
| Origin: | AgResearch, New Zealand |
| Sequenced by: | Sharik Khan, University of Illinois at Urbana-Champaign |
| Assembled by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Methylome called by: | William G. Alexande, Oak Ridge National Laboratory |
| Methyltransferases cloned by: | Adam Guss, Oak Ridge National Laboratory |
| MIC and Culturing Conditions by: | Sharik Khan, University of Illinois at Urbana-Champaign |
| Transformation by: |
Genome
Single contig of 2,084,047 bp.
DefenseFinder Results
Methylome
| #6mA modified motifs | |||
| #motif | methylCounts | totalCounts | %motifsMethyl |
| C(6mA)TCC | 2452 | 2922 | 83.92 |
| GATG(6mA)T | 2170 | 2556 | 84.90 |
| GG(6mA)TG | 2471 | 2922 | 84.57 |
| (6mA)GGNNNNNNRTC | 1037 | 1342 | 77.27 |
| G(6mA)YNNNNNNCCT | 984 | 1342 | 73.32 |
Methylating E. coli Strains
Culturing Conditions
Methanobrevibacter boviskoreani JH1T is a strict anaerobe and can be grown in both RMO2-medium and MSM (Methanobrevibacter smithii medium) topped with H2/CO2 (80:20, v/v). Growth of M. boviskoreani JH1T requires rumen fluid but does not need volatile fatty acids (VFAs). Upon plating, the strain forms colonies in 7-10 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Puromycin | |
| Nourseothricin | |
| Neomycin | |
| 8-ADP | |
| 5-Fluorouracil | |
| 2-BES | |
| 5-FOA |
