Methanobrevibacter ruminantium M1T: Difference between revisions
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{| class="wikitable" | {| class="wikitable" | ||
|Origin: | |Origin: | ||
| | |USA | ||
|- | |- | ||
|Sequenced by: | |Sequenced by: | ||
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== Methylome == | == Methylome == | ||
{| class="wikitable" | |||
|'''#motif''' | |||
|'''methylCounts''' | |||
|'''totalCounts''' | |||
|'''%motifsMethyl''' | |||
|- | |||
|CTGC(6mA)G | |||
| align="right" |2073 | |||
| align="right" |2194 | |||
| align="right" |94.48 | |||
|- | |||
|GATG(6mA)T | |||
| align="right" |3236 | |||
| align="right" |3369 | |||
| align="right" |96.05 | |||
|- | |||
|GGAR(6mA)G | |||
| align="right" |2810 | |||
| align="right" |2972 | |||
| align="right" |94.55 | |||
|- | |||
|G(5mC)GG | |||
| align="right" |3657 | |||
| align="right" |3756 | |||
| align="right" |97.36 | |||
|- | |||
|(5mC)CGC | |||
| align="right" |2004 | |||
| align="right" |3756 | |||
| align="right" |53.35 | |||
|} | |||
== Methylating ''E. coli'' == | == Methylating ''E. coli'' == | ||
| Line 40: | Line 70: | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''M. ruminantium'' M1 grows well in BY or RM02 medium (including formate (60 mM) and methanol (20 mM), co-enzymeM, vitamins and minerals pressurized with gas mix of H2:CO2 (80:20, v/v) and incubated at 39°C. Growth can be observed typically within 48 hours. On agar plates, ''M. ruminantium'' M1 requires a soft agar overlay as it will not grow on the agar surface. Agar plates must be incubated in canisters capable of pressurizing to 1 bar with H2:CO2 (80:20, v/v) gas mix. Titanium (III) NTA added to the media at concentrations of 0.125mM Ti III and 0.5mM NTA improved plating efficiency of M1. | |||
== Antimicrobial MIC Tests == | == Antimicrobial MIC Tests == | ||
{| class="wikitable" | |||
! rowspan="2" |Antibiotics | |||
! colspan="2" |Concentration | |||
! rowspan="2" |Observation Period | |||
|- | |||
|'''Liquid''' | |||
|'''Solid''' | |||
|- | |||
|Puromycin | |||
|2ug/mL | |||
|5ug/mL | |||
|~240 h | |||
|} | |||
== Protocols == | == Protocols == | ||
Latest revision as of 00:58, 27 October 2026
Introduction
Methanobrevibacter ruminantium M1 is the type strain of Methanobrevibacter genus isolated from bovine rumen contents [1].
Global Methane Hub Metadata
| Origin: | USA |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Guss Lab, ORNL |
| MIC and Culturing Conditions by: | Kay Pilkington |
| Transformation by: |
Genome
DefenseFinder Results
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Methylome
| #motif | methylCounts | totalCounts | %motifsMethyl |
| CTGC(6mA)G | 2073 | 2194 | 94.48 |
| GATG(6mA)T | 3236 | 3369 | 96.05 |
| GGAR(6mA)G | 2810 | 2972 | 94.55 |
| G(5mC)GG | 3657 | 3756 | 97.36 |
| (5mC)CGC | 2004 | 3756 | 53.35 |
Methylating E. coli
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Culturing Conditions
M. ruminantium M1 grows well in BY or RM02 medium (including formate (60 mM) and methanol (20 mM), co-enzymeM, vitamins and minerals pressurized with gas mix of H2:CO2 (80:20, v/v) and incubated at 39°C. Growth can be observed typically within 48 hours. On agar plates, M. ruminantium M1 requires a soft agar overlay as it will not grow on the agar surface. Agar plates must be incubated in canisters capable of pressurizing to 1 bar with H2:CO2 (80:20, v/v) gas mix. Titanium (III) NTA added to the media at concentrations of 0.125mM Ti III and 0.5mM NTA improved plating efficiency of M1.
Antimicrobial MIC Tests
| Antibiotics | Concentration | Observation Period | |
|---|---|---|---|
| Liquid | Solid | ||
| Puromycin | 2ug/mL | 5ug/mL | ~240 h |
Protocols
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Transformation Results
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External Links
Bryant MP (1965) Rumen methanogenic bacteria. In: Dougherty RW, Allen RS, Burroughs W, Jaskson NL, McGillard, eds. Physiology of digestion of the ruminant, Butterworths Publishing Inc., Washington, D.C.
