Methanobrevibacter ruminantium M1T
Introduction
Methanobrevibacter ruminantium M1 is the type strain of Methanobrevibacter genus isolated from bovine rumen contents [1].
Global Methane Hub Metadata
| Origin: | USA |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Guss Lab, ORNL |
| MIC and Culturing Conditions by: | Kay Pilkington |
| Transformation by: |
Genome
DefenseFinder Results
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Methylome
| #motif | methylCounts | totalCounts | %motifsMethyl |
| CTGC(6mA)G | 2073 | 2194 | 94.48 |
| GATG(6mA)T | 3236 | 3369 | 96.05 |
| GGAR(6mA)G | 2810 | 2972 | 94.55 |
| G(5mC)GG | 3657 | 3756 | 97.36 |
| (5mC)CGC | 2004 | 3756 | 53.35 |
Methylating E. coli
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Culturing Conditions
M. ruminantium M1 grows well in BY or RM02 medium (including formate (60 mM) and methanol (20 mM), co-enzymeM, vitamins and minerals pressurized with gas mix of H2:CO2 (80:20, v/v) and incubated at 39°C. Growth can be observed typically within 48 hours. On agar plates, M. ruminantium M1 requires a soft agar overlay as it will not grow on the agar surface. Agar plates must be incubated in canisters capable of pressurizing to 1 bar with H2:CO2 (80:20, v/v) gas mix. Titanium (III) NTA added to the media at concentrations of 0.125mM Ti III and 0.5mM NTA improved plating efficiency of M1.
Antimicrobial MIC Tests
| Antibiotics | Concentration | Observation Period | |
|---|---|---|---|
| Liquid | Solid | ||
| Puromycin | 2ug/mL | 5ug/mL | ~240 h |
Protocols
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Transformation Results
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External Links
Bryant MP (1965) Rumen methanogenic bacteria. In: Dougherty RW, Allen RS, Burroughs W, Jaskson NL, McGillard, eds. Physiology of digestion of the ruminant, Butterworths Publishing Inc., Washington, D.C.
