View source for Methanobrevibacter ruminantium M1T


== Introduction ==
''Methanobrevibacter ruminantium'' M1 is the type strain of Methanobrevibacter genus isolated from bovine rumen contents [1]. 

== Global Methane Hub Metadata ==
{| class="wikitable"
|Origin:
|USA
|-
|Sequenced by:
|Plasmidsaurus, ONT sequencing
|-
|Assembled by:
|William G. Alexander
|-
|Methylome called by:
|William G. Alexander
|-
|Methyltransferases cloned by:
|Guss Lab, ORNL
|-
|MIC and Culturing Conditions by:
|Kay Pilkington
|-
|Transformation by:
|
|}

== Genome ==
[[File:Methanobrevibacter ruminantium M1 genome assembly.png|center|thumb|'''Methanobrevibacter ruminantium M1 genome assembly.Source: Plasmidsaurus''']]

== DefenseFinder Results ==
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== Methylome ==
{| class="wikitable"
|'''#motif'''
|'''methylCounts'''
|'''totalCounts'''
|'''%motifsMethyl'''
|-
|CTGC(6mA)G
| align="right" |2073
| align="right" |2194
| align="right" |94.48
|-
|GATG(6mA)T
| align="right" |3236
| align="right" |3369
| align="right" |96.05
|-
|GGAR(6mA)G
| align="right" |2810
| align="right" |2972
| align="right" |94.55
|-
|G(5mC)GG
| align="right" |3657
| align="right" |3756
| align="right" |97.36
|-
|(5mC)CGC
| align="right" |2004
| align="right" |3756
| align="right" |53.35
|}

== Methylating ''E. coli'' ==
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== Culturing Conditions ==
''M. ruminantium'' M1 grows well in BY or RM02 medium (including formate (60 mM) and methanol (20 mM), co-enzymeM, vitamins and minerals pressurized with gas mix of H2:CO2 (80:20, v/v) and incubated at 39°C. Growth can be observed typically within 48 hours. On agar plates, ''M. ruminantium'' M1 requires a soft agar overlay as it will not grow on the agar surface. Agar plates must be incubated in canisters capable of pressurizing to 1 bar with H2:CO2 (80:20, v/v) gas mix. Titanium (III) NTA added to the media at  concentrations of  0.125mM Ti III and 0.5mM NTA improved plating efficiency of M1. 

== Antimicrobial MIC Tests ==
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== Protocols ==
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== Transformation Results ==
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== External Links ==
Bryant MP (1965) Rumen methanogenic bacteria. In: Dougherty RW, Allen RS, Burroughs W, Jaskson NL, McGillard, eds. Physiology of digestion of the ruminant, Butterworths Publishing Inc., Washington, D.C.