Lachnospiraceae bacterium NK3A20
Introduction
Lachnospiraceae bacterium NK3A20
Lachnospiraceae bacterium NK3A20 was isolated from sheep rumen contents in NZ [1] and is a representative of one of the most abundant bacterial taxa found in the rumen [2]. NK3A20 has an extensive complement of glycosyl hydrolases and can ferment a wide range of carbohydrates. Acetate, butyrate and hydrogen are the main products from glucose fermentation, while acetate is the main product from galacturonic or glucuronic acid fermentation [3]. Like Butyrivibrio sp., NK3A20 has two distinct respiratory enzyme complexes, Rnf and Ech.
The draft genome for NK3A20 (2,958,954 bp) was generated through the Hungate1000 project with JGI [2].
NCBI: Lachnospiraceae bacterium.
GTDB: CAG-791 sp900107575.
Risk group: 1.
Global Methane Hub Metadata
| Origin: | New Zealand, isolated by Nikki Kenters [1] |
| Sequenced by: | Sam Mahoney-Kurpe |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | |
| MIC and Culturing Conditions by: | |
| Transformation by: |
Genome
| Genome characteristics | |
|---|---|
| Contigs | 1 |
| size (bp) | 3,001,182 |
| GC % | 54.2 |
| CDS | 2513 |
| rRNA | 9 |
| tRNA | 46 |
| CRISPR | 1 |
DefenseFinder Results
Methylome
| Methylated motif | Percent methylated |
|---|---|
| C(6mA)YCNNNNNTGG | 99.1 |
| CC(6mA)NNNNNGRTG | 99.2 |
| C(5mC)GG | 76.5 |
Methylating E. coli Strains
Culturing Conditions
Antibiotic MIC Tests
Protocols
Transformation Results
External Links
[1]. Kenters N, Henderson G, Jeyanathan J, Kittelmann S, Janssen PH. 2011. Isolation of previously uncultured rumen bacteria by dilution to extinction using a new liquid culture medium. J Microbiol Methods 84: 52-60. doi: 10.1016/j.mimet.2010.10.011.
[2] Henderson G, Cox F, Ganesh S, Jonker A, Young W, Global Rumen Census Collaborators, Janssen PH. 2015. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep 5:14567. doi: 10.1038/srep14567.
[3] Kaminsky RA, Reid PM, Altermann E, Kenters N, Kelly WJ, Noel SJ, Attwood GT, Janssen PH. 2023. Rumen Lachnospiraceae isolate NK3A20 exhibits metabolic flexibility in response to substrate and coculture with a methanogen. Appl Environ Microbiol 89 :e0063423. doi: 10.1128/aem.00634-23.
[4] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
