Lachnospira multipara D32T
Introduction
Lachnospira multipara D32T
Lachnospira multipara D32T (DSM 3073) was isolated from bovine rumen contents, from an animal fed a ration of alfalfa hay and grain, in the USA [1]. D32 produces woolly colonies and ferments a range of carbohydrates producing acetate, formate, butyrate and ethanol along with CO2 and a small amount of hydrogen. Lachnospira multipara is regarded as one of the main rumen bacteria involved in pectin metabolism [2] and the D32 genome encodes pectin methylesterases predicted to produce methanol [3], a substrate for methylotrophic methanogens. D32 is the type species of the genus Lachnospira within the family Lachnospiraceae and the type stain of the L. multipara species [4].
The draft genome for D32 (2,871,860 bp) was generated through the Hungate1000 project with JGI [5]. It is likely that the D32 genome has a large plasmid that encodes Ech hydrogenase, hydrogenase maturation proteins and a cell-wall associated pectin methylesterase, similar to Butyrivibrio fibrisolvens D1.
LPSN: Lachnospira multipara corrig. Bryant and Small 1956.
NCBI: Lachnospira multipara.
GTDB: Lachnospira multipara.
Risk group: 1.
Global Methane Hub Metadata
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Genome
DefenseFinder Results
Methylome
Methylating E. coli Strains
Culturing Conditions
Antibiotic MIC Tests
Protocols
Transformation Results
External Links
[1] Bryant MP, Small N. 1956. Characteristics of two new genera of anaerobic curved rods isolated from the rumen of cattle. J Bacteriol 72: 22-26. doi: 10.1128/jb.72.1.22-26.1956.
[2] Dehority BA. 1969. Pectin-fermenting bacteria isolated from the bovine rumen. J Bacteriol 99: 189-96. doi: 10.1128/jb.99.1.189-196.1969.
[3] Kelly WJ, Leahy SC, Kamke J, Soni P, Koike S, Mackie R, Seshadri R, Cook GM, Morales SE, Greening C, Attwood GT. 2019. Occurrence and expression of genes encoding methyl-compound production in rumen bacteria. Anim Microbiome 1: 15. doi: 10.1186/s42523-019-0016-0.
[4] https://bacdive.dsmz.de/strain/6359.
[5] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
