Eubacterium sp. AB3007

From Global Methane Hub Wikipedia

Introduction

Eubacterium sp. AB3007

Eubacterium sp. AB3007 was isolated from the plant-adherent fraction of bovine rumen contents in NZ [1, cow B]. Its carbohydrate metabolism and fermentation end products have not been determined. It is a member of the family Anaerovoracaceae within the order Peptostreptococcales.

It is of interest as it is one of the few rumen bacteria whose genome encodes the gene cluster for the enzyme choline trimethylamine lyase (CTMAL; EC:4.3.99.4) which generates trimethylamine a substrate for methylotrophic methanogens, especially members of the Methanomassiliicoccales [2]. CTMAL is an enzyme complex composed of a catalytic choline utilisation polypeptide CutC, and an associated activating protein CutD, encoded by a 19 gene cluster that also contains genes encoding bacterial microcompartment proteins.

The draft genome for AB3007 (2,301,752 bp) was generated through the Hungate1000 project with JGI [3].

NCBI: Eubacterium sp.

GTDB: Aminicella sp000688015 (formerly Hornefia sp000688015)

Risk group: 1

Global Methane Hub Metadata

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Genome

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DefenseFinder Results

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Methylome

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Methylating E. coli

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Culturing Conditions

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Antimicrobial MIC Tests

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Protocols

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Transformation Results

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[1] Noel S. 2013. Cultivation and community composition analysis of plant-adherent rumen bacteria. PhD dissertation. Massey University, Palmerston North, New Zealand. https://mro.massey.ac.nz/server/api/core/bitstreams/bcfbe4cb-19a9-40c0-b5e8-61f9ddb90ce0/content.

[2] Kelly WJ, Leahy SC, Kamke J, Soni P, Koike S, Mackie R, Seshadri R, Cook GM, Morales SE, Greening C, Attwood GT. 2019. Occurrence and expression of genes encoding methyl-compound production in rumen bacteria. Anim Microbiome 1: 15. doi: 10.1186/s42523-019-0016-0.

[3] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.