Pseudobutyrivibrio ruminis A12-1T

From Global Methane Hub Wikipedia

Introduction

Pseudobutyrivibrio ruminis A12-1T

Pseudobutyrivibrio ruminis  A12-1T (DSM 9787) was isolated from bovine rumen contents in Sweden [1]. It stains Gram negative and ferments a range of carbohydrates producing butyrate, formate and lactate. It is the type species of the genus Pseudobutyrivibrio within the family Lachnospiraceae and the type stain of the P. ruminis species [2]

The draft genome for A12-1 (3,020,378 bp) was generated through the Hungate1000 project with JGI [3].

As with Butyrivibrio species A12-1has two distinct respiratory enzyme complexes, Rnf and Ech, which couple with two ATP synthases in separate chemiosmotic circuits and impact energy conservation in these organisms [4].

LPSN: Pseudobutyrivibrio ruminis van Gylswyk et al. 1996

NCBI: Pseudobutyrivibrio ruminis.

GTBD: Pseudobutyrivibrio ruminis.

Risk group: 1

Strain characteristics and function in the rumen

Strain history and information

Global Methane Hub Metadata

Strain origin: Sweden
Isolated from: Cow rumen
Sequenced by:
Assembled by: William G. Alexander
Genome and methylome analysis by:
MIC and culturing conditions by:
Transformed by:

Genome

P. ruminis A12-1T Genome Summary
Sequencing method Oxford Nanopore
Contigs
Base pairs
CDSs
CRISPR
Genes
rRNA
tRNA
Miscellaneous RNA
Antimicrobial resistance genes

Methylome

Methylome analysis summary results

P. ruminis A12-1T Methylome Summary
Isolation Motif % Motif Methylation

DefenseFinder Results

P. ruminis A12-1T DefenseFinder Results
DefenseFinder System # Found

Methylating Escherichia coli strains

E. coli strain AGXXXXX engineered to carry methytransferases X, X, X for methylating plasmid DNA for P. ruminis A12-1T.

Culturing Conditions

Antimicrobial Susceptibility

Transformation Results