Sharpea azabuensis KH1P5
Introduction
Sharpea azabuensis KH1P5
Sharpea azabuensis KH1P5 was isolated from bovine rumen contents in NZ using KH medium which was supplemented with haemin and vitamin K [1]. KH1P5 ferments glucose, fructose, galactose, cellobiose, sucrose, lactose and raffinose with D-lactate as the main fermentation product together with small amounts of acetate, formate and ethanol [2]. It is a member of the family Coprobacillaceae.
Sharpea and Kandleria were identified as key bacteria associated with the S-type (Sharpea-enriched) bacterial community, one of the two low-CH4 community types in rumen samples from sheep that differed in the amount of CH4 formed per unit of feed consumed when these sheep were fed pelletized lucerne [3]. A metagenomic and metatranscriptomic study conducted by Kamke et al. [4] confirmed that the relative abundance of Sharpea spp. was greater in a group of low-CH4 emitting sheep than in their high-CH4 emitting flock mates. It was hypothesized that lactate produced by these bacteria is further converted to butyrate by Megasphaera spp., resulting in lower CH4 emissions.
The draft genome for KH1P5 (2,329,465 bp) was generated through the Hungate1000 project with JGI [1]. Resequencing gave a single chromosome of 2,465,508 bp.
NCBI: Sharpea azabuensis.
GTDB: Sharpea azabuensis. KH1P5 groups with the type strain (ST18, DSM 18934) [5]
Risk group: 1
Sharpea azabuensis KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of extracellular polymeric substance (EPS) that requires removal via washing with chelating agents and detergent before work with the culture.
Global Methane Hub Metadata
| Origin: | New Zealand, Kelly Lab |
| Sequenced by: | Sam Mahoney Kurpe |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: | Roy Rodriguez Carrero |
Genome
DefenseFinder Results
Methylome
While S. azabuensis KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in E. coli strains, suggesting that they're vestigial defense systems.
Methylating E. coli Strains
Use a strain of E. coli that lacks dam, dcm, and hsdRMS, such as AG5589.
Culturing Conditions
S. azabuensis KH1P5 grows well in stock RCM at 37 °C.
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| chloramphenicol | 0.38 |
| erythromycin | 1 |
| kanamycin | insensitive |
| spectinomycin | 96 |
| tetracycline | 0.23 |
