Sharpea azabuensis KH1P5

S. azabuensis KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero

Introduction

Sharpea azabuensis KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of extracellular polymeric substance (EPS) that requires removal before work with the culture.

Methylome

While S. azabuensis KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in E. coli strains, suggesting that they're vestigial defense systems.

Methylating E. coli Strains

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Culturing Conditions

S. azabuensis KH1P5 grows well in stock RCM at 37 °C.

Antibiotic MIC Tests

MIC tests of S. azabuensis. Source: Dr. Roy Rodriguez Carrero

antibiotic	concentration (ug/mL)
chloramphenicol	0.38
erythromycin	1
kanamycin	resistant
spectinomycin	96
tetracycline	0.23

Protocols

• Removal of EPS via chelation washing