Genome
The genome of C. aminophilum FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka.
C. aminophilum FT genome with tRNA, rRNA, and CRISPR loci denoted
Methylome
motif
# sites methylated
total sites
% methylated
G(6mA)TCNNNNNGGT
1057
1060
99.72
(6mA)CCNNNNNGATC
1018
1060
96.04
GCC(6mA)NNNNNNNNTGT
191
197
96.45
AC(6mA)NNNNNNNNTGGC
186
197
94.42
CG(6mA)CNNNNNNTCG
191
191
100
CG(6mA)NNNNNNGTCG
190
191
99.48
ATTA(6mA)T
440
466
94.42
C(5mC)GCGG
3276
3450
94.96
Methylating E. coli strains
Culturing Conditions
Media
RCM (+cysteine) + Wolin's
RCM (+cysteine) +Wolin's +VFA
RCM +cysteine +Wolin's +VFA +TM
M2GSC
MDM
Time to Saturation
one overnight period
one overnight period
one overnight period
one overnight period
Not tested
Antibiotic MIC Tests
Raw photographic data of MIC tests on C. aminophilum FT. Source: Dr. Jill Walton
Antibiotic
Concentration Range
Tetracycline
12-16 ug/mL
Trimethoprim
Resistant
Ampicillin
0.25-0.38 ug/mL
Streptomycin
Resistant
Chloramphenicol
8-12 ug/mL
Gentamycin
32-48 ug/mL
Kanamycin
128-192 ug/mL
Erythromycin
0.094-0.19 ug/mL
Spectinomycin
Resistant
Protocols
External Links
