Wolin's Vitamin Solution
From Global Methane Hub Wikipedia
Description
Wolin's Vitamin Soution (commonly referred to as "Wolin's") is a common vitamin supplement for aerobic and anaerobic culturing of bacteria and archaea.
Recipe
| Compound | 500x Stock Concentration (g/L) | Working Concentration (g/L) |
|---|---|---|
| Biotin | 10 | 0.02 |
| Folic acid | 10 | 0.02 |
| Pyridoxine hydrochloride | 50 | 0.1 |
| Thiamine HCl | 25 | 0.05 |
| Riboflavin | 25 | 0.05 |
| Nicotinic acid | 25 | 0.05 |
| Calcium D-(+)-pantothenate | 25 | 0.05 |
| Vitamin B12 | 2 | 0.001 |
| p-aminobenzoic acid | 25 | 0.05 |
| (DL)-α-lipoic acid | 25 | 0.05 |
To produce a 500x stock, set 80% of water stirring on a plate and add vitamins sequentially. Once all have been added, add 10 M NaOH dropwise until the pH reaches ~7.5. Aliquot 40 mL of 500x solution into lightproof 50-mL conical vials. Store in the dark at -20 C.
