Ruminococcus flavefaciens AE3010
Introduction
Ruminococcus flavefaciens AE3010
Ruminococcus flavefaciens AE3010 is an obligately anaerobic, fibrolytic bacterium isolated from the rumen of a cow in New Zealand and selected as a key reference genome for the global Hungate1000 project [1]. Exhibiting the core phenotypic traits of the species, AE3010 relies on the breakdown of complex structural polysaccharides for growth, utilizing an extensive array of highly conserved cohesin and dockerin modules to assemble its specialized cell-surface cellulosome [2].
Global Methane Hub Metadata
| Origin: | United States of America, Macike lab |
| Sequenced by: | Makoto Adachi |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
| TGAA(6mA)T | 99.49 |
| CTTG(6mA)YG | 99.76 |
| CG(6mA)CC | 97.15 |
| CACA(6mA)T | 97.75 |
Methylating E. coli
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Culturing Conditions
R. flavefaciens AE3010 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. falvefaciens AE3010 on M10 agar is close to 66%.
Antimicrobial MIC Tests
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
| Antibiotic | Concentration (µg/ml) |
|---|---|
| Ampicilin | 0.5 |
| Chlorhamphenicol | 0.3 |
| Erythromycin | 1.6 |
| Gentamycin | 0.5 |
| Kanamycin | 4.7 |
| Streptomycin | 0.5 |
| Tetracycline | 0.1 |
Protocols
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Transformation Results
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External Links
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D, Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nat Biotechnol 36:359–367.
[2] Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT. 2024. A genomic analysis reveals the diversity of cellulosome displaying bacteria. Front Microbiol 15:1473396.
