Prevotella ruminicola 23T
Introduction
Prevotella ruminicola 23 (ATCC 19189, DSM 19721), also known as Xylanibacter ruminicola 23, is an obligately anaerobic, Gram-negative bacterium originally isolated from the bovine rumen [1]. It is the type strain of P. ruminicola and was originally described as Bacteroides ruminicola by Bryant et al. in 1958 [1]. The species was subsequently transferred to the genus Prevotella in 1990 [2].
P. ruminicola 23 contributes to the degradation of plant polysaccharides in the rumen, particularly xylan and other hemicelluloses. Its genome encodes numerous carbohydrate-active enzymes involved in the degradation of non-cellulosic polysaccharides [3]. Several xylan-degrading enzymes have been characterized, including a bifunctional xylanase/endoglucanase and a GH10 xylanase–ferulic acid esterase [4, 5].
The genome sequence of P. ruminicola 23 is available under GenBank accession CP002006 [3]. The species was reclassified as Xylanibacter ruminicola following a taxonomic revision of the genus Prevotella [6]. However, LPSN currently retains Prevotella ruminicola as the correct name.
LPSN: Prevotella ruminicola (Bryant et al. 1958) Shah and Collins 1990.
NCBI: Xylanibacter ruminicola (Bryant et al. 1958) Hitch et al. 2023.
Type strain: ATCC 19189 = DSM 19721 = JCM 8958 = Bryant 23.
Genome: CP002006.
Risk group: 2.
Global Methane Hub Metadata
Genome
Methylome
| Motif | Percent methylated |
|---|---|
| G(6mA)TC | 95.98 |
| CYC(6mA)NNNNNNNTCG/CG(6mA)NNNNNNNTGRG | 86.34/95.59 |
| GR(5mC)GYC | 95.15 |
| GG(5mC)C | 85.78 |
| AG(5mC)TC | 84.75 |
| GAG(5mC)T | 77.28 |
Culturing Conditions
Antimicrobial MIC Tests
External Links
[1] Bryant MP, Small N, Bouma C, Chu H. 1958. Bacteroides ruminicola n. sp. and Succinimonas amylolytica; the new genus and species; species of succinic acid-producing anaerobic bacteria of the bovine rumen. J Bacteriol 76:15–23. doi:10.1128/JB.76.1.15-23.1958.
[2] Shah HN, Collins DM. 1990. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int J Syst Bacteriol 40:205–208. doi:10.1099/00207713-40-2-205.
[3] Purushe J, Fouts DE, Morrison M, White BA, Mackie RI, et al. 2010. Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche. Microb Ecol 60:721–729. doi:10.1007/s00248-010-9692-8.
[4] Whitehead TR. 1993. Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria. Curr Microbiol 27:27–33. doi:10.1007/BF01576830.
[5] Dodd D, Kocherginskaya SA, Spies MA, Beery KE, Abbas CA, Mackie RI, Cann IKO. 2009. Biochemical analysis of a β-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J Bacteriol 191:3328–3338. doi:10.1128/JB.01628-08.
[6] Hitch TCA, Bisdorf K, Afrizal A, Riedel T, Overmann J, Strowig T, Clavel T. 2022. A taxonomic note on the genus Prevotella: Description of four novel genera and emended description of the genera Hallella and Xylanibacter. Syst Appl Microbiol 45:126354. doi:10.1016/j.syapm.2022.126354.
