Olsenella sp. KH2P3
Introduction
Tractidigestivibacter sp. KH2P3
Tractidigestivibacter sp. KH2P3 was isolated from bovine rumen contents in NZ using KH medium which was supplemented with haemin and vitamin K [1]. KH2P3 is a non-motile, non-spore forming Gram positive, anaerobe which produces D-lactate as the main product from glucose fermentation. KH2P3 is a high G+C lactic acid bacterium and while originally identified as Olsenella it was subsequently assigned to the genus Tractidigestivibacter within the family Atopobiaceae [2].
Tractidigestivibacter species encode glycyl radical enzyme (GRE) superfamily enzymes that catalyse reactions rarely found in other bacteria, such as aromatic amino acid decarboxylases that enable T. scatoligenes to produce skatole and p-cresol from tryptophan and tyrosine [3]. KH2P3 encodes two arylacetate decarboxylase gene clusters.
The draft genome for KH2P3 (2,421,020 bp) was generated through the Hungate1000 project with JGI [1].
NCBI: Olsenella sp.
GTDB: Tractidigestivibacter sp900119625
Risk group: 1.
Global Methane Hub Metadata
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Genome
DefenseFinder Results
Methylome
Methylating E. coli Strains
Culturing Conditions
Antibiotic MIC Tests
Protocols
Transformation Results
External Links
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
[2] Zgheib R, Anani H, Meng MM, Mailhe M, Ricaboni D, Morand A, Caputo A, Traore SI, Fontanini A, Armstrong N, Raoult D, Fournier PE, 2021. New human-associated species of the family Atopobiaceae and proposal to reclassify members of the genus Olsenella. Int J Syst Evol Microbiol 71. doi: 10.1099/ijsem.0.004819.
[3] Li X, Jensen RL, Højberg O, Canibe N, Jensen BB, 2015. Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces. Int J Syst Evol Microbiol 65, 1227-1233. doi: 10.1099/ijs.0.000083.
