Methanobrevibacter olleyae 1H5-1PT

Global Methane Hub Metadata

Origin:	AgResearch, New Zealand
Sequenced by:	Glen Cronan, University of Illinois at Urbana-Champaign
Assembled by:	Glen Cronan, University of Illinois at Urbana-Champaign
Methylome called by:	William G. Alexande, Oak Ridge National Laboratory
Methyltransferases cloned by:	N/A
MIC and Culturing Conditions by:	Glen Cronan, University of Illinois at Urbana-Champaign
Transformation by:	Sharik Khan, University of Illinois at Urbana-Champaign Komal Agrawal, University of Illinois at Urbana-Champaign

Genome

Single contig of 2,173,952 bp.

Methylome

No methylation motifs were detected in Methanobrevibacter olleyae 1H5-1PT.

Methylating E. coli Strains

No E. coli methylation strain has been constructed for Methanobrevibacter olleyae 1H5-1PT.

Culturing Conditions

M. olleyae 1H5-1PT is a strict anaerobe and grows well in both RMO2-medium and MSM (Methanobrevibacter smithii medium) topped with H₂/CO₂ (80:20, v/v). Growth of M. olleyae 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H₂/CO₂ (80:20) supplemented with 1000ppm H₂S .

MIC Tests

antibiotic	concentration (ug/mL)
Puromycin
Nourseothricin
Neomycin
8-ADP
5-Fluorouracil
2-BES
5-FOA

Protocols

• Removal of extracellular material via chelation washing