Ruminococcus albus 7

Scanning electron microscopic image of Ruminococcus albus 7. This photo belongs to the GMH Project and was taken by Kingsley Boateng.

Introduction

Ruminococcus albus 7

Ruminococcus albus (strain 7) is an obligately anaerobic, Gram-positive bacterium first isolated from the bovine rumen by Robert Edward Hungate in 1951 [1]. This species primarily utilizes plant-derived carbohydrates, such as cellulose, xylan, and cellobiose, as fermentation substrates, producing acetate and ethanol as its major end products [2].　Produces more ethanol, H₂, and CO₂ rather than Ruminococcus flavefaciens.

This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen [3]. Additioally, the production of H₂ during fermentation by R. albus, providing electrons for CO₂ reduction and methane production by methanogenic archaea [4]. Consequently, understanding the metabolic pathways and symbiotic relationships of R. albus 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock.

Global Methane Hub Metadata

Origin:	United States of America, Macike lab
Sequenced by:	Yuqi Li
Assembled by:	William G. Alexander
Methylome called by:	William G. Alexander
Methyltransferases cloned by:	Roy Rodriguez Carrero
MIC and Culturing Conditions by:	Roy Rodriguez Carrero
Transformation by:

Genome

Circos plot of R. albus 7. Source: Makoto Adachi

Methylome

Motif	Percent methylated
G(6mA)TC
GCGC(6mA)G
CAG(6mA)KC
CTYR(6mA)G
C(6mA)GNNNNNNNTTYG/ CRA(6mA)NNNNNNNCTG
G(6mA)STC
GAGG(6mA)G
G(5mC)WGC
GGN(5mC)C
CTC(4mC)TC

Methylating E. coli

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Culturing Conditions

R. albus 7 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. albus 7 on M10 agar is close to 100%.

Antimicrobial MIC Tests

Note: Antimicrobial MIC was assessed using M2GSC liquid medium.

Antibiotic	Concentration (µg/ml)
Ampicilin	2.92
Chlorhamphenicol	0.08
Erythromycin	1.79
Gentamycin	4.67
Kanamycin	5.77
Streptomycin	Resistance (>200)

Protocols

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Transformation Results

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External Links

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