Methanocatella gottschalkii HO
Introduction
Methanocatella gottschalkii HOT (formerly Methanobrevibacter gottschalkii) is a type strain isolated from the enrichment of horse faeces[1]. M. gottschalkii, along with Methanobrevibacter ruminantium, comprises about 74% of total archaeal OTUs from rumen samples collected across a wide range of geographical regions, hosts, and diets[2]. This universality makes them methanogens of particular interest to the Global Methane Hub, as it enables strategies to mitigate methane emissions by targeting these dominant methanogens.
Global Methane Hub Metadata
| Origin: | Leibniz Institute DSMZ, Germany |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | Christian Heryakusuma |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Christian Heryakusuma |
| Transformation by: |
Genome
| Assembly Summary | |
|---|---|
| Completeness | 99.97% |
| Contamination | 0.16% |
| Total contigs | 1 |
| Max contig length | 1,887,794 bp |
| GC content | 30.07% |
| Annotation Summary | |
|---|---|
| Coding density | 88.1% |
| tRNAs | 33 |
| tmRNAs | 0 |
| rRNAs | 7 |
| ncRNAs | 1 |
| ncRNAs regions | 1 |
| CRISPR arrays | 0 |
| CDSs | 1847 |
| Pseudogenes | 2 |
| Hypotheticals | 1585 |
| sORFs | 0 |
| Gaps | 0 |
| oriCs | 0 |
| oriVs | 0 |
| oriTs | 0 |
Genome was annotated using Bakta v1.12.0 with database v6.0, full (URL: github.com/oschwengers/bakta)[3].
DefenseFinder Results
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Methylome
| #motif | methylCounts | totalCounts | %motifsMethyl |
| C(6mA)YNNNNNRTTG | 1495 | 1558 | 95.96 |
| CA(6mA)YNNNNNRTG | 1522 | 1558 | 97.69 |
The methylome was analyzed using MIJAMP[4]. 4mC and 5mC methylation motifs were not present in this isolate.
Methylating E. coli
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Culturing Conditions
M. gottschalkii HO is cultivated in GMH-VT2 medium at 37°C and 50 rpm with a 30 psig gas mixture of H2:CO2 (80:20, v/v) as an energy substrate for 24-48 h. This methanogen can also be cultivated on GMH-VT2 plates using 1.5% gelrite or 2% agar as a gelling agent with a 12 to 30 psig H2:CO2 (80:20, v/v) gas mixture as an energy substrate. Visible growth on plate is usually observable within 2-5 days. The viability of M. gottschalkii HO on agar plates is 10-30 times higher than that of those grown on gelrite plates.
Antimicrobial MIC Tests
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Sodium sulfite | 0.5 mM | 0.3 mM |
| Mevinolin | 40 μM | 20 μM |
| 5-fluoroorotic acid (5-FOA) | Insensitive | Insensitive |
| 8-azahypoxanthine (8-AH) | Insensitive | Insensitive |
| 6-mercaptopurine (6-MP) | Insensitive | Insensitive |
| 5-fluorouracil (5-FU) | 0.25 mg/mL | 0.15 mg/mL |
| Sodium 2-bromoethanesulfonate (BES) | 2 mM | 1 mM |
Protocols
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Transformation Results
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External Links
- ↑ Miller, T. L. (2002). "Description of Methanobrevibacter gottschalkii sp. nov., Methanobrevibacter thaueri sp. nov., Methanobrevibacter woesei sp. nov. and Methanobrevibacter wolinii sp. nov". International Journal of Systematic and Evolutionary Microbiology. 52 (3): 819–822. https://doi.org/10.1099/00207713-52-3-819.
- ↑ Henderson, G., Cox, F., Ganesh, S. et al. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep 5, 14567 (2015). https://doi.org/10.1038/srep14567.
- ↑ Schwengers O, Jelonek L, Dieckmann MA, Beyvers S, Blom J, Goesmann A. Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification. Microb Genom. 2021 Nov;7(11):000685. doi: 10.1099/mgen.0.000685. PMID: 34739369; PMCID: PMC8743544.
- ↑ Alyssa K Tidwell, Evelyn Faust, Carrie A Eckert, Adam M Guss, William G Alexander, Discovering methylated DNA motifs in bacterial nanopore sequencing data with MIJAMP, Journal of Industrial Microbiology and Biotechnology, Volume 52, 2025, kuaf022, https://doi.org/10.1093/jimb/kuaf022.
