File:Rumen fluid, yeast extract and tryptone are all required for optimal growth of M. millerae SM9 .jpg
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M. millerae SM9 exhibited robust growth in the full MMSM (see Methods for composition), reaching a maximum OD600 of ~1.3 by ~160 hours. Omitting rumen fluid significantly limited growth and reduced the final OD600 to ~0.2. In contrast, removal of yeast extract or tryptone caused a moderate reduction, with OD600 peaking at ~0.6. When all three components were excluded, growth was severely limited, with OD600 remaining below 0.1 after 280 hours. Together, these data indicate that rumen fluid, yeast extract, and tryptone are all critical for optimal growth of M. millerae SM9.
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