MMSM
From Global Methane Hub Wikipedia
Description
Modified Methanobrevibacter smithii and Methanospheara stadtmanae medium (MMSM) is a rich, undefined medium commonly used for the anaerobic cultivation of Methanobrevibacter species.
Recipe
| Composition per 100 mL | Amount |
| Modified Rich medium for Methanobrevibacter (MMSM) | |
| Mineral 1 | 3.3 mL |
| Mineral 2 | 3.3 mL |
| Yeast extract | 0.2 g |
| Tryptone | 0.2 g |
| Na Acetate·3H2O (150 g/L) | 2 mL |
| Fe(NH4)2(SO4)2·6H2O (2 g/L) | 1 mL |
| Rezasurin (1 g/L) | 0.1 mL |
| Trace vitamins | 1 mL |
| Trace minerals | 1 mL |
| NaHCO3 | 0.5 g |
| Rumen fluid | 3 mL |
| NH4Cl | 0.1 g |
| L-Cysteine hydrochloride·H2O | 0.05 g |
| Na2S·9H2O | 0.1 mL |
| DTT | 0.05 g |
| ddH2O | 85 mL |
| Modification of MMSM for growth on formate | |
| Add sodium formate | 1.5 g |
| Add glycylglycine buffer, 1 M | 20 mL |
| ddH2O | 65 mL |
Note: some suppliers may replace up to 10 g of yeast extract with meat extract
Directions
Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.
Sources
- ThermoFisher CM0149 (Used by Guss Lab)
