Methanocatella millerae SM9
Introduction
Methanocatella millerae SM9 (formerly Methanobrevibacter millerae) was isolated from the rumen of a sheep fed a fresh forage diet[1]. Although initially identified as Methanobrevibacter sp. or M. smithii, SM9 shares 99% 16S rRNA gene sequence identity with the M. millerae type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species [2]. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub.
Global Methane Hub Metadata
| Origin: | Leibniz Institute DSMZ, Germany |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | Nana Shao |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing conditions by: | Nana Shao |
| Transformation by: |
Genome
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DefenseFinder Results
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Methylome
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Methylating E. coli
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Culturing Conditions
M. millerae SM9 is cultivated in MMSM medium at 37°C and 50 rpm with a 207 kPa of H2/CO2 (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same H2/CO2 as an energy substrate for 3-7 days.
Antimicrobial MIC Tests
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Puromycin | ||
| nourseothricin | μM | |
Protocols
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Transformation Results
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External Links
- ↑ 1. Joblin KN. (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. Australian Journal of Agricultural Research 50, 1307–1314. https://doi.org/10.1071/AR99004 2. Kelly WJ, Pacheco DM, Li D, Attwood GT, Altermann E, Leahy SC. The complete genome sequence of the rumen methanogen Methanobrevibacter millerae SM9. Stand Genomic Sci. 2016 Aug 17;11:49. doi: 10.1186/s40793-016-0171-9.
