Reinforced Clostridial Medium
From Global Methane Hub Wikipedia
Description
Reinforced Clostridial Medium (RCM) is a common rich undefined microbiological medium for the anaerobic culturing of microbes.
Recipe
| Compound | g/L |
|---|---|
| Yeast Extract | 13 |
| Peptone | 10 |
| Soluble Starch | 1 |
| Glucose | 5 |
| Cysteine HCl | 0.5 |
| Sodium Chloride | 5 |
| Sodium Acetate | 3 |
| Agar | 0.5 |
Note: some suppliers may replace up to 10 g of yeast extract with meat extract
Directions
Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.
Sources
- ThermoFisher CM0149 (Used by Guss Lab)
