Methanocatella gottschalkii HO
Introduction
Methanocatella gottschalkii HOT (formerly known as Methanobrevibacter gottschalkii) is a type strain isolated from the enrichment of horse faeces[1]. This methanogen, along with Methanobrevibacter ruminantium, comprises about 74% of total archaeal OTUs from rumen samples collected across geographical regions, hosts, and diets[2]. This universality makes them methanogens of utmost interest to the Global Methane Hub, as it could enable strategies to mitigate methane emissions by targeting only these dominant methanogens.
Global Methane Hub Metadata
| Origin: | Leibniz Institute DSMZ, Germany |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | Christian Heryakusuma |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Christian Heryakusuma |
| Transformation by: |
Genome
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DefenseFinder Results
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Methylome
| #motif | methylCounts | totalCounts | %motifsMethyl |
| C(6mA)YNNNNNRTTG | 1495 | 1558 | 95.96 |
| CA(6mA)YNNNNNRTG | 1522 | 1558 | 97.69 |
The methylome was analyzed using MIJAMP[3]. 4mC and 5mC methylation motifs were not present in this isolate.
Methylating E. coli
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Culturing Conditions
M. gottschalkii HO is cultivated in GMH-VT2 medium at 37°C and 50 rpm with a 30 psig gas mixture of H2:CO2 (80:20, v/v) as an energy substrate for 24-48 h. This methanogen can also be cultivated on GMH-VT2 plates using 1.5% gelrite or 2% agar as a gelling agent with a 12 to 30 psig H2:CO2 (80:20, v/v) gas mixture as an energy substrate for 2-5 days. The viability of M. gottschalkii on agar plates is 10-30 times higher than that of those grown on gelrite plates.
Antimicrobial MIC Tests
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Sodium sulfite | 0.5 mM | 0.3 mM |
| Mevinolin | 40 μM | 20 μM |
| 5-fluoroorotic acid (5-FOA) | Insensitive | Insensitive |
| 8-azahypoxanthine (8-AH) | Insensitive | Insensitive |
| 6-mercaptopurine (6-MP) | Insensitive | Insensitive |
| 5-fluorouracil (5-FU) | 0.25 mg/mL | 0.15 mg/mL |
| Sodium 2-bromoethanesulfonate (BES) | 2 mM | 1 mM |
Protocols
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Transformation Results
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External Links
- ↑ Miller, T. L. (2002). "Description of Methanobrevibacter gottschalkii sp. nov., Methanobrevibacter thaueri sp. nov., Methanobrevibacter woesei sp. nov. and Methanobrevibacter wolinii sp. nov". International Journal of Systematic and Evolutionary Microbiology. 52 (3): 819–822. https://doi.org/10.1099/00207713-52-3-819.
- ↑ Henderson, G., Cox, F., Ganesh, S. et al. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep 5, 14567 (2015). https://doi.org/10.1038/srep14567.
- ↑ Alyssa K Tidwell, Evelyn Faust, Carrie A Eckert, Adam M Guss, William G Alexander, Discovering methylated DNA motifs in bacterial nanopore sequencing data with MIJAMP, Journal of Industrial Microbiology and Biotechnology, Volume 52, 2025, kuaf022, https://doi.org/10.1093/jimb/kuaf022.
