Butyrivibrio fibrisolvens TB
Introduction
Butyrivibrio fibrisolvens TB
Butyrivibrio fibrisolvens TB (originally isolate PC/103) was isolated from bovine rumen contents in NZ on a medium containing the uronic acid-rich ' branched-B ' heteroglycan from red clover (Trifolium pratense) [1]. Like D1, TB ferments a broad range of complex plant polysaccharides with production of butyrate, formate, acetate and lactate.
The draft genome for TB (4,407,701 bp) was generated through the Hungate1000 project with JGI [2]. TB has a large plasmid encoding Ech and pectin methylesterase genes, similar to that found in D1.
NCBI: Butyrivibrio sp.
GTDB: Butyrivibrio fibrisolvens_C. Strain TB, along with several other isolates and MAGs, belongs in a different species group than D1.
Risk group: 1
(firmicutes)- Clostridia- Lachnospirales- Lachnospiraceae- Butyrivibrio
Genome
Methylome
| motif | # site methylated | total sites | % methylated |
|---|---|---|---|
| ATTA(6mA)T | Example | Example | Example |
| C(5mC)TGA | Example | Example | Example |
E.coli methylation strain
Completed, AG20809
Antibiotic MIC tests
MIC test strip result
| Antibiotic | MIC |
|---|---|
| Ampicillin | 0.016ug/ml |
| streptomycin | 6ug/ml |
| gentamicin | 0.38ug/ml |
| Spectinomycin | 2ug/ml |
| Chloramphenicol | 0.5ug/ml |
| Erythromycin | Resistant |
| Tetracycline | Resistant |
| kanamycin | Resistant |
Defensefinder prediction
- Predicted RM I, II, III is not listed
| Type / Subtype | Mechanism | Note |
|---|---|---|
| SspBCDE (SspBCDE) | Cut DNA without phosphorothioate modification | Lacks SspA & SspE (endonuclease) |
| HEC-02 (HEC-02) | ||
| Wadjet (Wadjet_I) | against closed-circular DNA (regardless of supercoiling state) | Lacks JetD (endonuclease) |
| AbiL (AbiL) | Against phage | |
| MazEF (MazEF) | Against phage | |
| AbiD (AbiD) | Against phage |
External Links
[1] Clarke RTJ, Bailey RW, Gaillard BDE. 1969. Growth of rumen bacteria on plant cell wall polysaccharides. J Gen Microbiol 56, 79-86. doi: 10.1099/00221287-56-1-79.
[2] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
