Volatile Fatty Acid Solution
From Global Methane Hub Wikipedia
Description
This Volatile Fatty Acid (VFA) solution is a supplement used in a variety of anaerobic media formulations.
Recipe
| Component | Volume for 5X Stock Solution |
|---|---|
| deionized water | 30 mL |
| isobutyric acid | 1 mL |
| n-valeric acid | 1 mL |
| isovaleric acid | 1 mL |
| DL-α-methylbutyric acid | 1 mL |
Add to a mixing vessel sequentially, pH to 7.0 with 1 M sodium hydroxide, then bring to a final volume of 100 mL with water. Filter sterilize and store at -20 °C.
