Ruminococcus albus 7
From Global Methane Hub Wikipedia
Introduction
Ruminococcus albus 7
Strictly anaerobic, Gram-positive, nonsporulating, nonmotile, chain-forming cocci. Found in human feces and rumen of cattle and other animals. Produces more ethanol, H2, and CO2 than Ruminococcus flavefaciens.
Global Methane Hub Metadata
| Origin: | United States of America, Macike lab |
| Sequenced by: | Makoto Adachi |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| G(6mA)TC | |
| GCGC(6mA)G | |
| CAG(6mA)KC | |
| CTYR(6mA)G | |
| C(6mA)GNNNNNNNTTYG/
CRA(6mA)NNNNNNNCTG |
|
| G(6mA)STC | |
| GAGG(6mA)G | |
| G(5mC)WGC | |
| GGN(5mC)C | |
| CTC(4mC)TC |
Methylating E. coli
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Culturing Conditions
R. albus 7 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. albus 7 on M10 agar is close to 100%.
Antimicrobial MIC Tests
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
| Antibiotic | Concentration (µg/ml) |
|---|---|
| Ampicilin | 2.92 |
| Chlorhamphenicol | 0.08 |
| Erythromycin | 1.79 |
| Gentamycin | 4.67 |
| Kanamycin | 5.77 |
| Streptomycin | Resistance (>200) |
Protocols
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Transformation Results
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External Links
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