Sharpea azabuensis KH1P5: Difference between revisions
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== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
Use a strain of ''E. coli'' that lacks dam, dcm, and hsdRMS, such as | Use a strain of ''E. coli'' that lacks dam, dcm, and hsdRMS, such as AG5589. | ||
== Culturing Conditions == | == Culturing Conditions == | ||
Revision as of 22:52, 19 October 2026
Introduction
Sharpea azabuensis KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of extracellular polymeric substance (EPS) that requires removal via washing with chelating agents and detergent before work with the culture.
Global Methane Hub Metadata
| Origin: | New Zealand, Kelly Lab |
| Sequenced by: | Sam Mahoney Kurpe |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: | Roy Rodriguez Carrero |
Genome
DefenseFinder Results
Methylome
While S. azabuensis KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in E. coli strains, suggesting that they're vestigial defense systems.
Methylating E. coli Strains
Use a strain of E. coli that lacks dam, dcm, and hsdRMS, such as AG5589.
Culturing Conditions
S. azabuensis KH1P5 grows well in stock RCM at 37 °C.
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| chloramphenicol | 0.38 |
| erythromycin | 1 |
| kanamycin | insensitive |
| spectinomycin | 96 |
| tetracycline | 0.23 |
