Sharpea azabuensis KH1P5: Difference between revisions

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Revision as of 20:02, 17 August 2026

S. azabuensis KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero

Introduction

Sharpea azabuensis KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of extracellular polymeric substance (EPS) that requires removal before work with the culture.

Global Methane Hub Metadata

Genome

DefenseFinder Results

Methylome

While S. azabuensis KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in E. coli strains, suggesting that they're vestigial defense systems.

Methylating E. coli Strains

xxxadd gene names tried here

Culturing Conditions

S. azabuensis KH1P5 grows well in stock RCM at 37 °C.

Antibiotic MIC Tests

MIC tests of S. azabuensis. Source: Dr. Roy Rodriguez Carrero
antibiotic concentration (ug/mL)
chloramphenicol 0.38
erythromycin 1
kanamycin resistant
spectinomycin 96
tetracycline 0.23



Protocols

Transformation Results