Sharpea azabuensis KH1P5: Difference between revisions
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[[File:Sabu tube.png|thumb|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero]] | |||
== Introduction == | |||
''Sharpea azabuensis'' KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance|extracellular polymeric substance (EPS)]] that requires removal before work with the culture. | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
| Line 6: | Line 11: | ||
== Methylome == | == Methylome == | ||
While ''S. azabuensis'' KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in ''E. coli'' strains, suggesting that they're vestigial defense systems. | |||
== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
xxxadd gene names tried here | |||
== Culturing Conditions == | == Culturing Conditions == | ||
''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. | |||
== Antibiotic MIC Tests == | == Antibiotic MIC Tests == | ||
[[File:Sabu MIC.png|left|thumb|MIC tests of ''S. azabuensis''. Source: Dr. Roy Rodriguez Carrero]] | |||
{| class="wikitable" | |||
!antibiotic | |||
!concentration (ug/mL) | |||
|- | |||
|chloramphenicol | |||
|0.38 | |||
|- | |||
|erythromycin | |||
|1 | |||
|- | |||
|kanamycin | |||
|resistant | |||
|- | |||
|spectinomycin | |||
|96 | |||
|- | |||
|tetracycline | |||
|0.23 | |||
|} | |||
== Protocols == | == Protocols == | ||
== Transformation == | * Removal of EPS via chelation washing | ||
== Transformation Results == | |||
== External Links == | == External Links == | ||
Revision as of 18:16, 17 August 2026
Introduction
Sharpea azabuensis KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of extracellular polymeric substance (EPS) that requires removal before work with the culture.
Global Methane Hub Metadata
Genome
DefenseFinder Results
Methylome
While S. azabuensis KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in E. coli strains, suggesting that they're vestigial defense systems.
Methylating E. coli Strains
xxxadd gene names tried here
Culturing Conditions
S. azabuensis KH1P5 grows well in stock RCM at 37 °C.
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| chloramphenicol | 0.38 |
| erythromycin | 1 |
| kanamycin | resistant |
| spectinomycin | 96 |
| tetracycline | 0.23 |
Protocols
- Removal of EPS via chelation washing
