Eubacterium maltosivorans SA11: Difference between revisions
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== Protocols == | == Protocols == | ||
* [[Eubacterium maltosivorans SA11 transformation protocol (BSI)]] | |||
== Transformation Results == | == Transformation Results == | ||
Latest revision as of 00:39, 1 November 2026
Introduction
Eubacterium maltosivorans SA11
Eubacterium maltosivorans SA11 (formerly Eubacterium limosum, and sheep acetogen SA11) was isolated from the rumen of a New Zealand sheep grazing fresh forage [1]. SA11 is a metabolically versatile bacterium able to grow on a wide variety of compounds including CO2/H2, formate, glucose, fructose, xylose, ribose, maltose, pyruvate, L-lactate, methanol, vanillate, syringate, and 3,4,5-trimethoxybenzoatehexoses. Acetate and butyrate are the main fermentation end products [2]. A feature of the SA11 genome is the presence of multiple copies of genes encoding trimethylamine methyltransferase family proteins (COG5598). SA11 has 39 genes in this category, but in most cases their substrate is not known. The genome contains a 55 kb prophage and genes required for the metabolism of 1,2 propanediol.
The SA11 genome consists of a single 4,150,332 bp circular chromosome [2].
LPSN: Eubacterium maltosivorans Feng et al. 2018
NCBI: Eubacterium maltosivorans
GTDB: Eubacterium maltosivorans. SA11 groups with the type strain of E. maltosivorans (Y1, DSM 105863) [3].
Risk group: 1
Global Methane Hub Metadata
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Genome
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DefenseFinder Results
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Methylome
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Methylating E. coli
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Culturing Conditions
SA11 grows well in M2G medium, with cultures reaching optical densities >2.5 after ~8 hours. Cells have a tendency to aggregate into clumps becoming more pronounced later in growth, complicating efforts to calculate plating efficiency. However, SA11 seems to plate relatively well on M2GSC agar (1.5% w/v) permitting selection of transformant colonies on this medium.
Antimicrobial MIC Tests
| antibiotic | MIC (μg/ml) |
|---|---|
| carbenicillin | 50 |
| clarithromycin | 5 |
| tetracycline | 5 |
| thiamphenicol | 5 |
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Protocols
Transformation Results
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External Links
[1] Joblin KN. 1999. Ruminal acetogens and their potential to lower ruminant methane emissions. Aust J Agric Res 50:1307–13. doi: 10.1071/AR99004.
[2] Kelly WJ, Henderson G, Pacheco DM, Li D, Reilly K, Naylor GE, Janssen PH, Attwood GT, Altermann E, Leahy SC. 2016. The complete genome sequence of Eubacterium limosum SA11, a metabolically versatile rumen acetogen. Stand Genomic Sci 11: 26. doi: 10.1186/s40793-016-0147-9
[3] Feng Y, Stams AJM, Sanchez-Andrea I, de Vos WM. 2018. Eubacterium maltosivorans sp. nov., a novel human intestinal acetogenic and butyrogenic bacterium with a versatile metabolism. Int J Syst Evol Microbiol 68: 3546–3550. doi: 10.1099/ijsem.0.003028.
