Ruminococcus albus 7: Difference between revisions
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'''''Ruminococcus albus 7''''' | '''''Ruminococcus albus 7''''' | ||
Ruminococcus albus (strain 7) is an obligately anaerobic, Gram-positive bacterium first isolated from the bovine rumen by Robert Edward Hungate in 1951 [1]. This species primarily utilizes plant-derived carbohydrates, such as cellulose, xylan, and cellobiose, as fermentation substrates, producing acetate and ethanol as its major end products [2]. Produces more ethanol, H<sub>2</sub>, and CO<sub>2</sub> rather than ''Ruminococcus flavefaciens.'' | |||
This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen [ | This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen [3]. Additioally, the production of H<sub>2</sub> during fermentation by ''R. albus'', providing electrons for CO₂ reduction and methane production by methanogenic archaea [4]. Consequently, understanding the metabolic pathways and symbiotic relationships of ''R. albus'' 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock. | ||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
Revision as of 01:47, 28 October 2026
Introduction
Ruminococcus albus 7
Ruminococcus albus (strain 7) is an obligately anaerobic, Gram-positive bacterium first isolated from the bovine rumen by Robert Edward Hungate in 1951 [1]. This species primarily utilizes plant-derived carbohydrates, such as cellulose, xylan, and cellobiose, as fermentation substrates, producing acetate and ethanol as its major end products [2]. Produces more ethanol, H2, and CO2 rather than Ruminococcus flavefaciens.
This bacterium have high cellolytic ability and play a key roll for initial fiber degradation in rumen [3]. Additioally, the production of H2 during fermentation by R. albus, providing electrons for CO₂ reduction and methane production by methanogenic archaea [4]. Consequently, understanding the metabolic pathways and symbiotic relationships of R. albus 7 is essential for ongoing research aimed at mitigating enteric methane emissions from livestock.
Global Methane Hub Metadata
| Origin: | United States of America, Macike lab |
| Sequenced by: | Yuqi Li |
| Assembled by: | William G. Alexander |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Roy Rodriguez Carrero |
| Transformation by: |
Genome
DefenseFinder Results
Methylome
| Motif | Percent methylated |
|---|---|
| G(6mA)TC | |
| GCGC(6mA)G | |
| CAG(6mA)KC | |
| CTYR(6mA)G | |
| C(6mA)GNNNNNNNTTYG/
CRA(6mA)NNNNNNNCTG |
|
| G(6mA)STC | |
| GAGG(6mA)G | |
| G(5mC)WGC | |
| GGN(5mC)C | |
| CTC(4mC)TC |
Methylating E. coli
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Culturing Conditions
R. albus 7 grown well in M2GSC and M10 medium at 37°C. Plating efficiency of R. albus 7 on M10 agar is close to 100%.
Antimicrobial MIC Tests
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
| Antibiotic | Concentration (µg/ml) |
|---|---|
| Ampicilin | 2.92 |
| Chlorhamphenicol | 0.08 |
| Erythromycin | 1.79 |
| Gentamycin | 4.67 |
| Kanamycin | 5.77 |
| Streptomycin | Resistance (>200) |
Protocols
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Transformation Results
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External Links
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