Methanobrevibacter olleyae YLM1: Difference between revisions
Created page with " == Introduction == ''Methanobrevibacter olleyae'' YLM1 was isolated from the rumen of a lamb<ref>Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: [https://www.sciencedirect.com/science/article/pii/S1075996404000496?via%3Dihub 10.1016/j.anaerobe.2004.05.003]. </ref>. Although initially identified a..." |
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== Introduction == | == Introduction == | ||
''Methanobrevibacter olleyae'' YLM1 was isolated from the rumen of a lamb<ref>Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: [https://www.sciencedirect.com/science/article/pii/S1075996404000496?via%3Dihub 10.1016/j.anaerobe.2004.05.003]. </ref>. Although initially identified as ''Methanobrevibacter'' sp. or ''Methanobrevibacter smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the ''Methanocatella (Methanobrevibacter) millerae'' type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species<ref>Kelly WJ, Li D, Lambie SC, Cox F, Attwood GT, Altermann E, Leahy SC. Draft Genome Sequence of the Rumen Methanogen ''Methanobrevibacter olleyae'' YLM1. Genome Announc. 2016 Apr 7;4(2):e00232-16. doi: [https://journals.asm.org/doi/10.1128/genomea.00232-16 10.1128/genomeA.00232-16].</ref>. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub. | ''Methanobrevibacter olleyae'' YLM1 was a hydrogenotrophic methanogen isolated from the rumen of a lamb<ref>Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: [https://www.sciencedirect.com/science/article/pii/S1075996404000496?via%3Dihub 10.1016/j.anaerobe.2004.05.003]. </ref>. Although initially identified as ''Methanobrevibacter'' sp. or ''Methanobrevibacter smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the ''Methanocatella (Methanobrevibacter) millerae'' type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species<ref>Kelly WJ, Li D, Lambie SC, Cox F, Attwood GT, Altermann E, Leahy SC. Draft Genome Sequence of the Rumen Methanogen ''Methanobrevibacter olleyae'' YLM1. Genome Announc. 2016 Apr 7;4(2):e00232-16. doi: [https://journals.asm.org/doi/10.1128/genomea.00232-16 10.1128/genomeA.00232-16].</ref>. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub. | ||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
Revision as of 21:09, 20 October 2026
Introduction
Methanobrevibacter olleyae YLM1 was a hydrogenotrophic methanogen isolated from the rumen of a lamb[1]. Although initially identified as Methanobrevibacter sp. or Methanobrevibacter smithii, SM9 shares 99% 16S rRNA gene sequence identity with the Methanocatella (Methanobrevibacter) millerae type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species[2]. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub.
Global Methane Hub Metadata
| Origin: | Lucidome Bio, NZ |
| Sequenced by: | Plasmidsaurus, ONT + Illumina |
| Assembled by: | Nana Shao |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing conditions by: | Nana Shao |
| Transformation by: |
Genome
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DefenseFinder Results
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Methylome
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Methylating E. coli
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Culturing Conditions
M. millerae SM9 is cultivated in Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM) at 37°C. To ensure good gas transfer to the media, tubes are incubated on their sides (or at a shallow angle so that the media does not contact the stoppers) with gentle shaking at 50 rpm and 207 kPa of H2/CO2 (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same pressure of H2/CO2 as an energy substrate for 3-7 days.
Antimicrobial MIC Tests
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Puromycin MIC determination in M. millerae SM9. Source: Dr. Nana Shao
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Puromycin | ||
| nourseothricin | μM | |
Protocols
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Transformation Results
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External Links
- ↑ Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: 10.1016/j.anaerobe.2004.05.003.
- ↑ Kelly WJ, Li D, Lambie SC, Cox F, Attwood GT, Altermann E, Leahy SC. Draft Genome Sequence of the Rumen Methanogen Methanobrevibacter olleyae YLM1. Genome Announc. 2016 Apr 7;4(2):e00232-16. doi: 10.1128/genomeA.00232-16.
