Methanocatella millerae SM9: Difference between revisions
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== Introduction == | == Introduction == | ||
''Methanocatella millerae'' SM9 (formerly ''Methanobrevibacter millerae'') was isolated from the rumen of a sheep fed a fresh forage diet<ref>Joblin KN. (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. ''Australian Journal of Agricultural Research'' 50, 1307–1314. [https://connectsci.au/cp/article/50/8/1307/33473/Ruminal-acetogens-and-their-potential-to-lower https://doi.org/10.1071/AR99004]</ref>. Although initially identified as ''Methanobrevibacter'' sp. or ''M. smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the '' | ''Methanocatella millerae'' SM9 (formerly ''Methanobrevibacter millerae'') was isolated from the rumen of a sheep fed a fresh forage diet<ref>Joblin KN. (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. ''Australian Journal of Agricultural Research'' 50, 1307–1314. [https://connectsci.au/cp/article/50/8/1307/33473/Ruminal-acetogens-and-their-potential-to-lower https://doi.org/10.1071/AR99004]</ref>. Although initially identified as ''Methanobrevibacter'' sp. or ''M. smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the ''Methanobrevibacter millerae'' type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species<ref>Kelly WJ, Pacheco DM, Li D, Attwood GT, Altermann E, Leahy SC. The complete genome sequence of the rumen methanogen ''Methanobrevibacter millerae'' SM9. Stand Genomic Sci. 2016 Aug 17;11:49. doi: [https://link.springer.com/article/10.1186/s40793-016-0171-9 10.1186/s40793-016-0171-9].</ref>. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub. | ||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
Revision as of 18:28, 15 October 2026
Introduction
Methanocatella millerae SM9 (formerly Methanobrevibacter millerae) was isolated from the rumen of a sheep fed a fresh forage diet[1]. Although initially identified as Methanobrevibacter sp. or M. smithii, SM9 shares 99% 16S rRNA gene sequence identity with the Methanobrevibacter millerae type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species[2]. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub.
Global Methane Hub Metadata
| Origin: | Leibniz Institute DSMZ, Germany |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | Nana Shao |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing conditions by: | Nana Shao |
| Transformation by: |
Genome
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DefenseFinder Results
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Methylome
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Methylating E. coli
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Culturing Conditions
M. millerae SM9 is cultivated in MMSM medium at 37°C and 50 rpm with a 207 kPa of H2/CO2 (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same H2/CO2 as an energy substrate for 3-7 days.
Antimicrobial MIC Tests
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Puromycin | ||
| nourseothricin | μM | |
Protocols
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Transformation Results
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External Links
- ↑ Joblin KN. (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. Australian Journal of Agricultural Research 50, 1307–1314. https://doi.org/10.1071/AR99004
- ↑ Kelly WJ, Pacheco DM, Li D, Attwood GT, Altermann E, Leahy SC. The complete genome sequence of the rumen methanogen Methanobrevibacter millerae SM9. Stand Genomic Sci. 2016 Aug 17;11:49. doi: 10.1186/s40793-016-0171-9.
