Methanocatella gottschalkii HO: Difference between revisions
From Global Methane Hub Wikipedia
Heryakusuma (talk | contribs) No edit summary |
Heryakusuma (talk | contribs) No edit summary |
||
| Line 50: | Line 50: | ||
| align="right" |97.69 | | align="right" |97.69 | ||
|} | |} | ||
The methylome was analyzed using MIJAMP<ref>Alyssa K Tidwell, Evelyn Faust, Carrie A Eckert, Adam M Guss, William G Alexander, Discovering methylated DNA motifs in bacterial nanopore sequencing data with MIJAMP, ''Journal of Industrial Microbiology and Biotechnology'', Volume 52, 2025, kuaf022, https://doi.org/10.1093/jimb/kuaf022. </ref>. 4mC and 5mC methylation motifs were not present in this isolate. | |||
== Methylating ''E. coli'' == | == Methylating ''E. coli'' == | ||
| Line 55: | Line 56: | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''M. gottschalkii'' HO is cultivated in GMH-VT2 medium at 37°C and 50 rpm with a 30 psig gas mixture of H<sub>2</sub>:CO<sub>2</sub> (80:20, v/v) as an energy substrate. | |||
== Antimicrobial MIC Tests == | == Antimicrobial MIC Tests == | ||
{| class="wikitable" | |||
|+ | |||
! rowspan="2" |Antibiotics | |||
! colspan="2" |Concentration | |||
|- | |||
|'''Liquid''' | |||
|'''Solid''' | |||
|- | |||
|Sodium sulfite | |||
|0.5 mM | |||
| | |||
|- | |||
|Mevinolin | |||
|40 μM | |||
| | |||
|- | |||
|5-fluoroorotic acid (5-FOA) | |||
|Insensitive | |||
| | |||
|- | |||
|8-azahypoxanthine (8-AH) | |||
|Insensitive | |||
| | |||
|- | |||
|6-mercaptopurine (6-MP) | |||
|Insensitive | |||
| | |||
|- | |||
|5-fluorouracil (5-FU) | |||
|0.25 mg/mL | |||
|0.15 mg/mL | |||
|- | |||
|Sodium 2-bromoethanesulfonate (BES) | |||
|2 mM | |||
| | |||
|} | |||
== Protocols == | == Protocols == | ||
Revision as of 20:48, 14 October 2026
Introduction
add here
Global Methane Hub Metadata
| Origin: | Leibniz Institute DSMZ, Germany |
| Sequenced by: | Plasmidsaurus, ONT sequencing |
| Assembled by: | Christian Heryakusuma |
| Methylome called by: | William G. Alexander |
| Methyltransferases cloned by: | Roy Rodriguez Carrero |
| MIC and Culturing Conditions by: | Christian Heryakusuma |
| Transformation by: |
Genome
add here
DefenseFinder Results
add here
Methylome
| #motif | methylCounts | totalCounts | %motifsMethyl |
| C(6mA)YNNNNNRTTG | 1495 | 1558 | 95.96 |
| CA(6mA)YNNNNNRTG | 1522 | 1558 | 97.69 |
The methylome was analyzed using MIJAMP[1]. 4mC and 5mC methylation motifs were not present in this isolate.
Methylating E. coli
add here
Culturing Conditions
M. gottschalkii HO is cultivated in GMH-VT2 medium at 37°C and 50 rpm with a 30 psig gas mixture of H2:CO2 (80:20, v/v) as an energy substrate.
Antimicrobial MIC Tests
| Antibiotics | Concentration | |
|---|---|---|
| Liquid | Solid | |
| Sodium sulfite | 0.5 mM | |
| Mevinolin | 40 μM | |
| 5-fluoroorotic acid (5-FOA) | Insensitive | |
| 8-azahypoxanthine (8-AH) | Insensitive | |
| 6-mercaptopurine (6-MP) | Insensitive | |
| 5-fluorouracil (5-FU) | 0.25 mg/mL | 0.15 mg/mL |
| Sodium 2-bromoethanesulfonate (BES) | 2 mM | |
Protocols
add here
Transformation Results
add here
External Links
add here
- ↑ Alyssa K Tidwell, Evelyn Faust, Carrie A Eckert, Adam M Guss, William G Alexander, Discovering methylated DNA motifs in bacterial nanopore sequencing data with MIJAMP, Journal of Industrial Microbiology and Biotechnology, Volume 52, 2025, kuaf022, https://doi.org/10.1093/jimb/kuaf022.
