Reinforced Clostridial Medium: Difference between revisions
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Alexanderwg (talk | contribs) Created page with "== Recipe == {| class="wikitable" |+ !Compound !g/L |- |Yeast Extract |13 |- |Peptone |10 |- |Soluble Starch |1 |- |Glucose |5 |- |Cysteine HCl |0.5 |- |Sodium Chloride |5 |- |Sodium Acetate |3 |- |Agar |0.5 |} Note: some suppliers may replace up to 10 g of yeast extract with meat extract == Sources == * [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab)" |
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== Description == | |||
Reinforced Clostridial Medium (RCM) is a common rich undefined microbiological medium for the anaerobic culturing of microbes. | |||
== Recipe == | == Recipe == | ||
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Note: some suppliers may replace up to 10 g of yeast extract with meat extract | Note: some suppliers may replace up to 10 g of yeast extract with meat extract | ||
=== Directions === | |||
Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes. | |||
== Sources == | == Sources == | ||
* [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab) | * [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab) | ||
Revision as of 17:41, 23 July 2026
Description
Reinforced Clostridial Medium (RCM) is a common rich undefined microbiological medium for the anaerobic culturing of microbes.
Recipe
| Compound | g/L |
|---|---|
| Yeast Extract | 13 |
| Peptone | 10 |
| Soluble Starch | 1 |
| Glucose | 5 |
| Cysteine HCl | 0.5 |
| Sodium Chloride | 5 |
| Sodium Acetate | 3 |
| Agar | 0.5 |
Note: some suppliers may replace up to 10 g of yeast extract with meat extract
Directions
Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.
Sources
- ThermoFisher CM0149 (Used by Guss Lab)
