Methanobrevibacter olleyae 1H5-1PT: Difference between revisions
From Global Methane Hub Wikipedia
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== Methylome == | == Methylome == | ||
No methylation motifs were detected in '' | No methylation motifs were detected in ''Methanobrevibacter olleyae'' 1H5-1PT. | ||
== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
No ''E. coli'' methylation strain has been constructed for '' | No ''E. coli'' methylation strain has been constructed for ''Methanobrevibacter olleyae'' 1H5-1PT. | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''M. olleyae'' 1H5-1PT grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubators filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S . | |||
== | == MIC Tests == | ||
{| class="wikitable" | {| class="wikitable" | ||
!antibiotic | !antibiotic | ||
!concentration (ug/mL) | !concentration (ug/mL) | ||
|- | |- | ||
| | |Puromycin | ||
| | | | ||
|- | |- | ||
| | |Nourseothricin | ||
| | | | ||
|- | |- | ||
| | |Neomycin | ||
| | | | ||
|- | |- | ||
| | |8-ADP | ||
| | | | ||
|- | |- | ||
| | |5-Fluorouracil | ||
| | | | ||
|- | |- | ||
| | |2-BES | ||
| | | | ||
|- | |- | ||
| | |5-FOA | ||
| | | | ||
|} | |} | ||
Revision as of 18:13, 28 October 2026
Introduction
Global Methane Hub Metadata
| Origin: | AgResearch, New Zealand |
| Sequenced by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Assembled by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Methylome called by: | William G. Alexande, Oak Ridge National Laboratory |
| Methyltransferases cloned by: | N/A |
| MIC and Culturing Conditions by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Transformation by: | Sharik Khan, University of Illinois at Urbana-Champaign
Komal Agrawal, University of Illinois at Urbana-Champaign |
Genome
DefenseFinder Results
Methylome
No methylation motifs were detected in Methanobrevibacter olleyae 1H5-1PT.
Methylating E. coli Strains
No E. coli methylation strain has been constructed for Methanobrevibacter olleyae 1H5-1PT.
Culturing Conditions
M. olleyae 1H5-1PT grows well in both RMO2-medium and MSM (Methanobrevibacter smithii medium) topped with H2/CO2 (80:20, v/v). Growth of M. olleyae 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubators filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .
MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Puromycin | |
| Nourseothricin | |
| Neomycin | |
| 8-ADP | |
| 5-Fluorouracil | |
| 2-BES | |
| 5-FOA |
