MMSM: Difference between revisions
From Global Methane Hub Wikipedia
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| == Description ==
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| Modified ''Methanobrevibacter smithii'' and ''Methanospheara stadtmanae'' medium (MMSM) is a rich, undefined medium commonly used for the anaerobic cultivation of ''Methanobrevibacter'' species.
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| == Recipe ==
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| {| class="wikitable"
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| |'''Composition per 100 mL'''
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| |'''Amount'''
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| |-
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| |''Modified Rich medium for Methanobrevibacter (MMSM)''
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| |-
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| |Mineral 1
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| |3.3 mL
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| |-
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| |Mineral 2
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| |3.3 mL
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| |-
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| |Yeast extract
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| |0.2 g
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| |-
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| |Tryptone
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| |0.2 g
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| |-
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| |Na Acetate·3H<sub>2</sub>O (150 g/L)
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| |2 mL
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| |-
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| |Fe(NH<sub>4</sub>)<sub>2</sub>(SO<sub>4</sub>)<sub>2</sub>·6H<sub>2</sub>O (2 g/L)
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| |1 mL
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| |-
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| |Rezasurin (1 g/L)
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| |0.1 mL
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| |-
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| |Trace vitamins
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| |1 mL
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| |-
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| |Trace minerals
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| |1 mL
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| |-
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| |NaHCO<sub>3</sub>
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| |0.5 g
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| |-
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| |Rumen fluid
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| |3 mL
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| |-
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| |NH<sub>4</sub>Cl
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| |0.1 g
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| |-
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| |L-Cysteine hydrochloride·H<sub>2</sub>O
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| |0.05 g
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| |-
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| |Na<sub>2</sub>S·9H<sub>2</sub>O
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| |0.1 mL
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| |-
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| |DTT
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| |0.05 g
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| |-
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| |ddH<sub>2</sub>O
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| |85 mL
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| |-
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| |''Modification of MMSM for growth on formate''
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| |-
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| |Add sodium formate
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| |1.5 g
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| |-
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| |Add glycylglycine buffer, 1 M
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| |20 mL
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| |-
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| |ddH<sub>2</sub>O
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| |65 mL
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| |}
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| Note: some suppliers may replace up to 10 g of yeast extract with meat extract
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| === Directions ===
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| Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.
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| == Sources ==
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| * [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab)
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Latest revision as of 18:19, 16 October 2026
