Wolin's Vitamin Solution: Difference between revisions
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Alexanderwg (talk | contribs) Created page with "== Description == Wolin's Vitamin Soution (commonly referred to as "Wolin's") is a common vitamin supplement for aerobic and anaerobic culturing of bacteria and archaea. == Recipe == {| class="wikitable" |+ !Compound !500x Stock Concentration (g/L) !Working Concentration (g/L) |- |Biotin |10 |0.02 |- |Folic acid |10 |0.02 |- |Pyridoxine hydrochloride |50 |0.1 |- |Thiamine HCl |25 |0.05 |- |Riboflavin |25 |0.05 |- |Nicotinic acid |25 |0.05 |- |Calcium D-(+)-pantothenate..." |
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To produce a 500x stock, set 80% of water stirring on a plate and add vitamins sequentially. Once all have been added, add 10 M NaOH dropwise until the pH reaches ~7.5. Aliquot 40 mL of 500x solution into lightproof 50-mL conical vials. Store in the dark at -20 C. | To produce a 500x stock, set 80% of water stirring on a plate and add vitamins sequentially. Once all have been added, add 10 M NaOH dropwise until the pH reaches ~7.5. Aliquot 40 mL of 500x solution into lightproof 50-mL conical vials. Store in the dark at -20 C. | ||
== Resources == | |||
* Wolin, F. A., M. J. Wolin, and R. S. Wolfe. (1963) Formation of methane by bacterial extracts. J. Biol. Chem. 238:2882-2886. | |||
Latest revision as of 18:03, 23 July 2026
Description
Wolin's Vitamin Soution (commonly referred to as "Wolin's") is a common vitamin supplement for aerobic and anaerobic culturing of bacteria and archaea.
Recipe
| Compound | 500x Stock Concentration (g/L) | Working Concentration (g/L) |
|---|---|---|
| Biotin | 10 | 0.02 |
| Folic acid | 10 | 0.02 |
| Pyridoxine hydrochloride | 50 | 0.1 |
| Thiamine HCl | 25 | 0.05 |
| Riboflavin | 25 | 0.05 |
| Nicotinic acid | 25 | 0.05 |
| Calcium D-(+)-pantothenate | 25 | 0.05 |
| Vitamin B12 | 2 | 0.001 |
| p-aminobenzoic acid | 25 | 0.05 |
| (DL)-α-lipoic acid | 25 | 0.05 |
To produce a 500x stock, set 80% of water stirring on a plate and add vitamins sequentially. Once all have been added, add 10 M NaOH dropwise until the pH reaches ~7.5. Aliquot 40 mL of 500x solution into lightproof 50-mL conical vials. Store in the dark at -20 C.
Resources
- Wolin, F. A., M. J. Wolin, and R. S. Wolfe. (1963) Formation of methane by bacterial extracts. J. Biol. Chem. 238:2882-2886.
