Clostridium aminophilum FT: Difference between revisions

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== Genome ==
== Genome ==
The genome of ''C. aminophilum'' FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka.
[[File:Cami genome.jpg|alt=A representation of the C. aminophilum FT genome.|center|''C. aminophilum'' F<sup>T</sup> genome with tRNA, rRNA, and CRISPR loci annotated. Source: Dr. William G. Alexander|frame]]


== Methylome ==
== Methylome ==
{| class="wikitable"
!motif
!# sites methylated
!total sites
!% methylated
|-
|G(6mA)TCNNNNNGGT
|1057
|1060
|99.72
|-
|(6mA)CCNNNNNGATC
|1018
|1060
|96.04
|-
|GCC(6mA)NNNNNNNNTGT
|191
|197
|96.45
|-
|AC(6mA)NNNNNNNNTGGC
|186
|197
|94.42
|-
|CG(6mA)CNNNNNNTCG
|191
|191
|100
|-
|CG(6mA)NNNNNNGTCG
|190
|191
|99.48
|-
|ATTA(6mA)T
|440
|466
|94.42
|-
|C(5mC)GCGG
|3276
|3450
|94.96
|}


== Methylating ''E. coli'' strains ==
== Methylating ''E. coli'' strains ==


== Culturing Conditions ==
== Culturing Conditions ==
{| class="wikitable"
|+
|Media
|[[Reinforced Clostridial Medium|RCM]] (+cysteine) + [[Wolin's Vitamin Solution|Wolin's]]
|RCM (+cysteine) +[[Wolin's Vitamin Solution|Wolin's]] +[[Volatile Fatty Acid Solution|VFA]]
|RCM +cysteine +[[Wolin's Vitamin Solution|Wolin's]] +[[Volatile Fatty Acid Solution|VFA]] +TM
|M2GSC
|MDM
|-
|Time to Saturation
|one overnight period
|one overnight period
|one overnight period
|one overnight period
|Not tested
|}
== Antibiotic MIC Tests ==
[[File:Cami MIC.jpg|left|thumb|Raw photographic data of MIC tests on ''C. aminophilum'' FT. Source: Dr. Jill Walton]]
{| class="wikitable sortable mw-collapsible mw-collapsed"
!Antibiotic
!Concentration Range
|-
|Tetracycline
|12-16 ug/mL
|-
|Trimethoprim
|Resistant
|-
|Ampicillin
|0.25-0.38 ug/mL
|-
|Streptomycin
|Resistant
|-
|Chloramphenicol
|8-12 ug/mL
|-
|Gentamycin
|32-48 ug/mL
|-
|Kanamycin
|128-192 ug/mL
|-
|Erythromycin
|0.094-0.19 ug/mL
|-
|Spectinomycin
|Resistant
|}


== Protocols ==
== Protocols ==
== External Links ==

Latest revision as of 18:41, 14 October 2026

Global Methane Hub Metadata

Genome

The genome of C. aminophilum FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka.

A representation of the C. aminophilum FT genome.
C. aminophilum FT genome with tRNA, rRNA, and CRISPR loci annotated. Source: Dr. William G. Alexander


Methylome

motif # sites methylated total sites % methylated
G(6mA)TCNNNNNGGT 1057 1060 99.72
(6mA)CCNNNNNGATC 1018 1060 96.04
GCC(6mA)NNNNNNNNTGT 191 197 96.45
AC(6mA)NNNNNNNNTGGC 186 197 94.42
CG(6mA)CNNNNNNTCG 191 191 100
CG(6mA)NNNNNNGTCG 190 191 99.48
ATTA(6mA)T 440 466 94.42
C(5mC)GCGG 3276 3450 94.96

Methylating E. coli strains

Culturing Conditions

Media RCM (+cysteine) + Wolin's RCM (+cysteine) +Wolin's +VFA RCM +cysteine +Wolin's +VFA +TM M2GSC MDM
Time to Saturation one overnight period one overnight period one overnight period one overnight period Not tested

Antibiotic MIC Tests

Raw photographic data of MIC tests on C. aminophilum FT. Source: Dr. Jill Walton
Antibiotic Concentration Range
Tetracycline 12-16 ug/mL
Trimethoprim Resistant
Ampicillin 0.25-0.38 ug/mL
Streptomycin Resistant
Chloramphenicol 8-12 ug/mL
Gentamycin 32-48 ug/mL
Kanamycin 128-192 ug/mL
Erythromycin 0.094-0.19 ug/mL
Spectinomycin Resistant

Protocols

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