Streptococcus equinus B315: Difference between revisions
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== Methylome == | == Methylome == | ||
{| class="wikitable" | |||
| valign="top" |'''#6mA modified motifs''' | |||
| valign="top" | | |||
| valign="top" | | |||
| valign="top" | | |||
|- | |||
| valign="top" |'''#motif''' | |||
| valign="top" |methylCounts | |||
| valign="top" |totalCounts | |||
| valign="top" |%motifsMethyl | |||
|- | |||
| valign="top" |'''GAR(6mA)NNNNNNNTAC''' | |||
| valign="top" |412 | |||
| valign="top" |430 | |||
| valign="top" |95.81 | |||
|- | |||
| valign="top" |'''GT(6mA)NNNNNNNTYTC''' | |||
| valign="top" |413 | |||
| valign="top" |430 | |||
| valign="top" |96.05 | |||
|} | |||
== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
== Culturing Conditions == | == Culturing Conditions == | ||
''Streptococcus bovis'' JB1 grows on both M2GSC and [[SDM culture medium|SDM]] (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO2 and 5% H2. Similarly, ''Streptococcus bovis'' JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO2 and 5% H2. | |||
== Antibiotic MIC Tests == | == Antibiotic MIC Tests == | ||
[[File:S. equinus MIC.jpg|left|thumb|274x274px|MIC test of ''Streptococcus'' ''equinus'' B315]] | |||
{| class="wikitable" | |||
!antibiotic | |||
!concentration (ug/mL) | |||
|- | |||
|Ampicillin | |||
|0.44 | |||
|- | |||
|Kanamycin | |||
|Resistant (>200 ug/mL) | |||
|- | |||
|Chloramphenicol | |||
|0.49 | |||
|- | |||
|Tetracycline | |||
|1 | |||
|- | |||
|Gentamicin | |||
|11.1 | |||
|- | |||
|Streptomycin | |||
|Resistant (>200 ug/mL) | |||
|- | |||
|Erythromycin | |||
|1 | |||
|} | |||
== Protocols == | == Protocols == | ||
* '''[[SDM culture medium|Preparation of SDM culture medium]]''' | |||
* '''Preparation of M2GSC culture medium''' | |||
* [[Streptococcus transformation protocol|'''Transformation protocol''']] | |||
== Transformation Results == | == Transformation Results == | ||
Latest revision as of 18:47, 29 October 2026
Introduction
Streptococcus equinus B315
Streptococcus equinus B315 was isolated from bovine rumen contents in NZ [1]. B315 is proteolytic, Gram positive, and able to ferment a wide range of carbohydrates. Its main fermentation product is lactate, together with succinate and acetate [1]. The B315 genome encodes two lantibiotic bacteriocin biosynthetic gene clusters.
The draft genome for B315 (1,786,673 bp) was generated through the Hungate1000 project with JGI [2].
NCBI: Streptococcus equinus.
GTDB: Streptococcus equinus. B315 belongs to the same species group as the S. equinus type strain (DSM 20558).
Risk group: 2.
Global Methane Hub Metadata
| Origin: | New Zealand |
| Sequenced by: | Ahmed Hetta and Yuqi Li |
| Assembled by: | William Alexander |
| Methylome called by: | William Alexander |
| Methyltransferases cloned by: | |
| MIC and Culturing Conditions by: | |
| Transformation by: | Ahmed Hetta |
Genome
DefenseFinder Results
Methylome
| #6mA modified motifs | |||
| #motif | methylCounts | totalCounts | %motifsMethyl |
| GAR(6mA)NNNNNNNTAC | 412 | 430 | 95.81 |
| GT(6mA)NNNNNNNTYTC | 413 | 430 | 96.05 |
Methylating E. coli Strains
Culturing Conditions
Streptococcus bovis JB1 grows on both M2GSC and SDM (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO2 and 5% H2. Similarly, Streptococcus bovis JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO2 and 5% H2.
Antibiotic MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Ampicillin | 0.44 |
| Kanamycin | Resistant (>200 ug/mL) |
| Chloramphenicol | 0.49 |
| Tetracycline | 1 |
| Gentamicin | 11.1 |
| Streptomycin | Resistant (>200 ug/mL) |
| Erythromycin | 1 |
Protocols
- Preparation of SDM culture medium
- Preparation of M2GSC culture medium
Transformation Results
External Links
[1] Attwood GT, Reilly K. 1995. Identification of proteolytic rumen bacteria isolated from New Zealand cattle. J Appl Bacteriol 79: 22-9. doi: 10.1111/j.1365-2672.1995.tb03119.x.
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
