Methanobrevibacter olleyae 1H5-1PT: Difference between revisions
From Global Methane Hub Wikipedia
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== Genome == | == Genome == | ||
Single contig of 2,173,952 bp. | |||
== DefenseFinder Results == | == DefenseFinder Results == | ||
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== Culturing Conditions == | == Culturing Conditions == | ||
''M. olleyae'' 1H5-1PT grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe | ''M. olleyae'' 1H5-1PT is a strict anaerobe and grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S . | ||
== MIC Tests == | == MIC Tests == | ||
Latest revision as of 18:46, 28 October 2026
Introduction
Global Methane Hub Metadata
| Origin: | AgResearch, New Zealand |
| Sequenced by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Assembled by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Methylome called by: | William G. Alexande, Oak Ridge National Laboratory |
| Methyltransferases cloned by: | N/A |
| MIC and Culturing Conditions by: | Glen Cronan, University of Illinois at Urbana-Champaign |
| Transformation by: | Sharik Khan, University of Illinois at Urbana-Champaign
Komal Agrawal, University of Illinois at Urbana-Champaign |
Genome
Single contig of 2,173,952 bp.
DefenseFinder Results
Methylome
No methylation motifs were detected in Methanobrevibacter olleyae 1H5-1PT.
Methylating E. coli Strains
No E. coli methylation strain has been constructed for Methanobrevibacter olleyae 1H5-1PT.
Culturing Conditions
M. olleyae 1H5-1PT is a strict anaerobe and grows well in both RMO2-medium and MSM (Methanobrevibacter smithii medium) topped with H2/CO2 (80:20, v/v). Growth of M. olleyae 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .
MIC Tests
| antibiotic | concentration (ug/mL) |
|---|---|
| Puromycin | |
| Nourseothricin | |
| Neomycin | |
| 8-ADP | |
| 5-Fluorouracil | |
| 2-BES | |
| 5-FOA |
