Clostridium aminophilum FT: Difference between revisions
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== Methylating ''E. coli'' strains == | == Methylating ''E. coli'' strains == | ||
== Culturing Conditions == | == Culturing Conditions == | ||
Latest revision as of 18:41, 14 October 2026
Global Methane Hub Metadata
Genome
The genome of C. aminophilum FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka.
Methylome
| motif | # sites methylated | total sites | % methylated |
|---|---|---|---|
| G(6mA)TCNNNNNGGT | 1057 | 1060 | 99.72 |
| (6mA)CCNNNNNGATC | 1018 | 1060 | 96.04 |
| GCC(6mA)NNNNNNNNTGT | 191 | 197 | 96.45 |
| AC(6mA)NNNNNNNNTGGC | 186 | 197 | 94.42 |
| CG(6mA)CNNNNNNTCG | 191 | 191 | 100 |
| CG(6mA)NNNNNNGTCG | 190 | 191 | 99.48 |
| ATTA(6mA)T | 440 | 466 | 94.42 |
| C(5mC)GCGG | 3276 | 3450 | 94.96 |
Methylating E. coli strains
Culturing Conditions
| Media | RCM (+cysteine) + Wolin's | RCM (+cysteine) +Wolin's +VFA | RCM +cysteine +Wolin's +VFA +TM | M2GSC | MDM |
| Time to Saturation | one overnight period | one overnight period | one overnight period | one overnight period | Not tested |
Antibiotic MIC Tests
| Antibiotic | Concentration Range |
|---|---|
| Tetracycline | 12-16 ug/mL |
| Trimethoprim | Resistant |
| Ampicillin | 0.25-0.38 ug/mL |
| Streptomycin | Resistant |
| Chloramphenicol | 8-12 ug/mL |
| Gentamycin | 32-48 ug/mL |
| Kanamycin | 128-192 ug/mL |
| Erythromycin | 0.094-0.19 ug/mL |
| Spectinomycin | Resistant |
